Generation of integration-free and region-specific neural progenitors from primate fibroblasts.
Generation of integration-free and region-specific neural progenitors from primate fibroblasts.
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DOI：
10.1016/j.celrep.2013.04.004
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发表时间：
2013-05-30
期刊：
影响因子：
8.8
通讯作者：
Zhang SC
中科院分区：
文献类型：
--
作者：
Lu J;Liu H;Huang CT;Chen H;Du Z;Liu Y;Sherafat MA;Zhang SC
将出生后和成年人和猴成纤维细胞用含有Yamanaka因子的仙台病毒感染24小时，然后将它们在含有白血病抑制因子（LIF）、转化生长因子（TGF）-β抑制剂SB 431542和糖原合成酶激酶（GSK）-3β抑制剂CHIR 99021的化学成分确定的培养基中在39°C下培养以灭活病毒。诱导的神经祖细胞（iNP）集落早在第13天就出现，并且可以扩增>20代。在相同的限定条件下，在37° C或39°C下均未形成诱导多能干细胞（iPSC）集落。iNP主要表达后脑基因并分化成后脑神经元，并且当尾化时，它们产生富集的脊髓运动神经元群体。移植到前脑后，iNP衍生的细胞保留了后脑的身份。在具有可预测命运选择的限定条件下从成年灵长类动物成纤维细胞产生限定的、无整合的iNP的能力将促进疾病建模和治疗开发。
Postnatal and adult human and monkey fibroblasts were infected with Sendai virus containing the Yamanaka factors for 24 hr, then they were cultured in a chemically defined medium containing leukemia inhibitory factor (LIF), transforming growth factor (TGF)-β inhibitor SB431542, and glycogen synthase kinase (GSK)-3β inhibitor CHIR99021 at 39°C for inactivation of the virus. Induced neural progenitor (iNP) colonies appeared as early as day 13 and can be expanded for >20 passages. Under the same defined condition, no induced pluripotent stem cell (iPSC) colonies formed at either 37°Cor 39°C. The iNPs predominantly express hindbrain genes and differentiate into hindbrain neurons, and when caudalized, they produced an enriched population of spinal motor neurons. Following transplantation into the forebrain, the iNP-derived cells retained the hindbrain identity. The ability to generate defined, integration-free iNPs from adult primate fibroblasts under a defined condition with predictable fate choices will facilitate disease modeling and therapeutic development.
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影响因子：
64.8
作者：
Kriks, Sonja;Shim, Jae-Won;Piao, Jinghua;Ganat, Yosif M.;Wakeman, Dustin R.;Xie, Zhong;Carrillo-Reid, Luis;Auyeung, Gordon;Antonacci, Chris;Buch, Amanda;Yang, Lichuan;Beal, M. Flint;Surmeier, D. James;Kordower, Jeffrey H.;Tabar, Viviane;Studer, Lorenz
通讯作者：
Studer, Lorenz
DOI：
10.1126/science.1162494
发表时间：
2008-11-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Stadtfeld M;Nagaya M;Utikal J;Weir G;Hochedlinger K
通讯作者：
Hochedlinger K
DOI：
10.1073/pnas.1121003109
发表时间：
2012-02-14
影响因子：
11.1
作者：
Lujan, Ernesto;Chanda, Soham;Wernig, Marius
通讯作者：
Wernig, Marius
影响因子：
23.9
作者：
Han, Dong Wook;Tapia, Natalia;Schoeler, Hans R.
通讯作者：
Schoeler, Hans R.
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya