TUMOR-NECROSIS-FACTOR AND CARDIAC-FUNCTION
TUMOR-NECROSIS-FACTOR AND CARDIAC-FUNCTION
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DOI：
10.1097/00005373-199206000-00003
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发表时间：
1992-06-01
影响因子：
--
通讯作者：
GAMELLI, RL
中科院分区：
文献类型：
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作者：
DEMEULES, JE;PIGULA, FA;GAMELLI, RL
采用离体乳头肌技术和改良的Langendorff制备方法，测定了肿瘤坏死因子（TNF）（活化巨噬细胞的产物）对心肌性能的直接影响。在雄性成年大鼠接受100 ng/kg TNF（A组）或100 μ g/kg TNF（B组）或盐水（对照）后4-5小时，从它们获得乳头肌。与对照组相比，B组动物表现出显著更大的峰值张力发展和收缩速度（p < 0.05）。在A组动物中，这些变量与对照组的变量没有显著差异（p > 0.05）。电生理学测量显示，与对照组相比，A组和B组动物的静息膜电位显著降低（p < 0.05）。与对照组相比，用TNF处理18-22小时前动物的血清灌注的整个心脏显示出显著的收缩性损害、收缩压发展速率降低和舒张速率降低（p < 0.05）。两组灌注心脏的冠状动脉流量和心肌含水量相似。这些数据表明，肿瘤坏死因子刺激对心肌功能的早期有益作用，18-22小时后与心肌性能受损相关。这种效应似乎是血清可转移的。
The direct effect of tumor necrosis factor (TNF), a product of activated macrophages, on myocardial performance was determined using an isolated papillary muscle technique and a modified Langendorff preparation. Papillary muscle was obtained from male adult rats 4-5 hours after they received either 100 ng/kg TNF (group A), or 100-mu-g/kg TNF (group B) or saline (control). Group B animals exhibited significantly greater peak tension development and velocity of contraction compared with controls (p < 0.05). In group A animals these variables were not significantly different from those of the controls (p > 0.05). Electrophysiologic measurements revealed a significant decrease in resting membrane potential in both group A and group B animals compared with the controls (p < 0.05). Whole hearts perfused with serum from animals treated with TNF 18-22 hours earlier exhibited significant impairment of contractility, decreased rate of systolic pressure development, and decreased rate of relaxation compared with the controls (p < 0.05). Coronary flow and myocardial water content were similar for both groups of perfused hearts. These data suggest that tumor necrosis factor stimulates an early beneficial effect on myocardial function, which 18-22 hours later is associated with impairment of myocardial performance. This effect appears to be serum transferable.