Contributions of cellular and humoral immunity of Galleria mellonella larvae in defence against oral infection by Bacillus thuringiensis
Contributions of cellular and humoral immunity of Galleria mellonella larvae in defence against oral infection by Bacillus thuringiensis
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DOI：
10.1016/j.jip.2014.04.003
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发表时间：
2014-06-01
影响因子：
3.4
通讯作者：
Glupov, V. V.
中科院分区：
文献类型：
--
作者：
Grizanova, E. V.;Dubovskiy, I. M.;Glupov, V. V.
本文研究了大蜡螟Galleria mellonella在口服苏云金杆菌引起的细菌感染过程中的细胞和体液免疫反应。研究了两种不同的剂量强度以评估免疫参数对诱导的Bt抗性的贡献。低剂量（亚致死LC15）感染导致血淋巴酚氧化酶和溶菌酶样活性显着升高，增强血细胞的吞噬活性，并在感染后48和72小时感染的幼虫增加包囊反应。较高剂量的Bt（半致死LC 50）也引发显着升高血淋巴酚氧化酶和溶菌酶样活性，但降低凝血指数和酚氧化酶活性的感染幼虫的血细胞。在这两种类型的感染中，循环血细胞池被耗尽。本文讨论了细胞和体液免疫反应在诱导昆虫对肠道细菌感染Bt的抗性中的重要性。(C)2014爱思唯尔公司All rights reserved.
In this study the cellular and humoral immune reactions of the Greater wax moth Galleria mellonella have been investigated during bacterial infection caused by oral administration of Bacillus thuringiensis. Two different dose strengths were investigated to assess the contribution of immune parameters to induced Bt resistance. Low-dose (sublethal LC15) infection resulted in significantly elevated haemolymph phenoloxidase and lysozyme-like activity, enhanced phagocytic activity of haemocytes, and increased encapsulation responses in infected larvae at 48 and 72 h post infection. Higher doses of Bt (half-lethal LC50) also triggered significantly elevated haemolymph phenoloxidase and lysozyme-like activity, but decreased the coagulation index and activity of phenoloxidase in haemocytes of infected larvae. In both types of infection, the pool of circulating haemocytes became depleted. The importance of cellular and humoral immune reactions in induced insect resistance to intestinal bacterial infection Bt is herein discussed. (C) 2014 Elsevier Inc. All rights reserved.