High human cytomegalovirus loads and diverse linked variable genotypes in both HIV-1 infected and exposed, but uninfected, children in Africa
High human cytomegalovirus loads and diverse linked variable genotypes in both HIV-1 infected and exposed, but uninfected, children in Africa
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DOI：
10.1016/j.virol.2008.09.001
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发表时间：
2008-12-05
期刊：
影响因子：
3.7
通讯作者：
Gompels, U. A.
中科院分区：
文献类型：
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作者：
Bates, M.;Monze, M.;Gompels, U. A.
在非洲HIV-1/AIDS流行地区赞比亚，采用实时定量PCR分析了HIV-1感染、暴露（HIV-1+母亲）或未感染人群中有症状或无症状的儿科队列中的人巨细胞病毒（HCMV）。在94%的HIV-1+呼吸道儿童死亡样本中鉴定出HCMV感染，50%的样本具有10（3）-10（8）拷贝/10（6）细胞的高DNA载量。相比之下，HCMV病毒血症与高DNA载量，表明急性感染，在10%的住院发热婴儿，50%的HIV-1+。而高血清载量在1%的无症状婴儿中，2%的HIV-1+，在HIV-1感染或暴露但阴性的婴儿中水平更高。显示了所有8种连锁高变糖蛋白gN-gO基因型，包括鉴定出具有gO 5连锁的新gN 4d组（之前仅Merlin参考菌株）和具有多重感染的样本。总的来说，这显示了非洲的全球基因型（与一些疱疹病毒不同）和暴露于HIV-1+但未感染的婴儿（一个新兴群体）的急性儿科HCMV感染。(C)2008年爱思唯尔公司All rights reserved.
Human cytomegalovirus, HCMV, was analysed using real-time quantitative PCR in symptomatic or asymptomatic pediatric cohorts from HIV-1 infected, exposed (HIV-1+ mothers), or uninfected groups in Zambia, an HIV-1/AIDS endemic region of Africa. HCMV infections were identified in 94% samples from HIV-1+ respiratory pediatric mortalities, 50% with high DNA loads of 10(3)-10(8) copies/10(6) cells. In comparison, HCMV viremia with high DNA loads, indicative of acute infections, were in 10% hospitalised febrile infants, with 50% HIV-1+. Whereas high sera loads were in 1% of asymptomatic infants, with 2% HIV-1+, and higher levels in both HIV-1 infected or exposed, but negative infants. All 8 linked-hypervariable glycoprotein gN-gO genotypes were shown, including identification of a new gN4d group with gO5 linkage (previously only Merlin reference strain), and samples with multiple infections. Overall, this shows global genotypes in Africa (unlike some herpesviruses) and acute pediatric HCMV infections in both HIV-1+ plus exposed, but uninfected infants, an emerging group. (C) 2008 Elsevier Inc. All rights reserved.