Comparison of functional proteomic analyses of human breast cancer cell lines T47D and MCF7.
Comparison of functional proteomic analyses of human breast cancer cell lines T47D and MCF7.
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DOI：
10.1371/journal.pone.0031532
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lin SX
中科院分区：
文献类型：
--
作者：
Aka JA;Lin SX
T47 D和MCF 7是两种人乳腺癌细胞系，其广泛用作体外和体内（肿瘤异种移植物）乳腺癌研究的实验模型。通过蛋白质组学分析，在这些细胞系中鉴定了参与癌症发展的几种蛋白质。虽然这些研究报道了每个细胞系的蛋白质组学谱，但到目前为止，它们的差异蛋白质表达谱尚未建立。在这里，我们使用二维凝胶和质谱分析来比较两种细胞系T47 D和MCF 7的蛋白质组谱。我们的数据显示，超过164个蛋白质在它们之间差异表达。根据它们的生物学功能，结果表明，参与细胞生长刺激、抗凋亡机制和癌发生的蛋白质在T47 D中比在MCF 7中更强地表达。这些蛋白质包括G1/S特异性细胞周期蛋白D3和抑制素。相反，与T47 D相比，涉及转录抑制和凋亡调节的蛋白质，包括转录抑制因子NF-X1、腈水解酶同源物2和白细胞介素-10，在MCF 7中更强烈地表达。发现先前描述为乳腺癌生物标志物的五种蛋白质，即组织蛋白酶D、组织蛋白酶B、蛋白S100-A14、热休克蛋白β-1（HSP 27）和增殖细胞核抗原（PCNA）在两种细胞系中差异表达。结果表明，T47 D和MCF 7之间存在差异表达，为进一步研究乳腺癌的发生机制提供了有用的信息。
T47D and MCF7 are two human hormone-dependent breast cancer cell lines which are widely used as experimental models for in vitro and in vivo (tumor xenografts) breast cancer studies. Several proteins involved in cancer development were identified in these cell lines by proteomic analyses. Although these studies reported the proteomic profiles of each cell line, until now, their differential protein expression profiles have not been established. Here, we used two-dimensional gel and mass spectrometry analyses to compare the proteomic profiles of the two cell lines, T47D and MCF7. Our data revealed that more than 164 proteins are differentially expressed between them. According to their biological functions, the results showed that proteins involved in cell growth stimulation, anti-apoptosis mechanisms and cancerogenesis are more strongly expressed in T47D than in MCF7. These proteins include G1/S-specific cyclin-D3 and prohibitin. Proteins implicated in transcription repression and apoptosis regulation, including transcriptional repressor NF-X1, nitrilase homolog 2 and interleukin-10, are, on the contrary, more strongly expressed in MCF7 as compared to T47D. Five proteins that were previously described as breast cancer biomarkers, namely cathepsin D, cathepsin B, protein S100-A14, heat shock protein beta-1 (HSP27) and proliferating cell nuclear antigen (PCNA), are found to be differentially expressed in the two cell lines. A list of differentially expressed proteins between T47D and MCF7 was generated, providing useful information for further studies of breast cancer mechanisms with these cell lines as models.
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影响因子：
2
作者：
Sarvaiya, Hetal A.;Yoon, Jung H.;Lazar, Iulia M.
通讯作者：
Lazar, Iulia M.
影响因子：
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作者：
Aka, Juliette A.;Mazumdar, Mausumi;Lin, Sheng-Xiang
通讯作者：
Lin, Sheng-Xiang
影响因子：
13.3
作者：
Blamey, R;Collins, J;Volpe, A
通讯作者：
Volpe, A
影响因子：
7.8
作者：
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通讯作者：
KORNFELD, S
影响因子：
7.4
作者：
Havlis, J;Thomas, H;Shevchenko, A
通讯作者：
Shevchenko, A