Atomic force microscopy in histology and cytology.
Atomic force microscopy in histology and cytology.
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组织学和细胞学中的原子力显微镜。
DOI：
10.1679/aohc.59.421
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发表时间：
1996
影响因子：
--
通讯作者：
M. Shigeno
中科院分区：
文献类型：
--
作者：
T. Ushiki;J. Hitomi;S. Ogura;T. Umemoto;M. Shigeno
本文简要介绍了原子力显微镜(AFM)的原理，并展示了我们在生物样品中应用原子力显微镜的结果。原子力显微镜发明于1986年，是一种用锋利的探头追踪样品表面形貌的仪器，同时监测探头和样品表面之间的相互作用力。因此，AFM提供了高分辨率的样品的三维表面图像。对于生物学家来说，原子力显微镜的优点是可以在非真空(即空气或液体)环境中可视化非导电材料。该质粒DNA的AFM图像与使用旋转阴影技术的透射电子显微镜的图像相当，并且具有无需染色或涂层即可直接检查分子的优点。非接触式原子力显微镜在空气中显示的人类中期染色体和小鼠胶原纤维的表面结构与扫描电子显微镜所获得的结构相似。关于结构高度的定量信息可以进一步从AFM图像中获得。无包埋的薄组织切片可用于原子力显微镜观察细胞内结构。本综述还展示了在液体中以接触方式收集的活体培养细胞的AFM图像。这项技术为我们提供了高分辨率的细胞运动的三维观察。尽管原子力显微镜成像有一些先天的局限性，但原子力显微镜在组织学和细胞学中提供有价值的新信息方面具有巨大的潜力。
This review briefly introduces the principles of the atomic force microscope (AFM) and shows our own results of AFM application to biological samples. The AFM, invented in 1986, is an instrument that traces the surface topography of the sample with a sharp probe while monitoring the interaction forces working between the probe and sample surface. Thus, the AFM provides three-dimensional surface images of the sample with high resolution. The advantage of the AFM for biologists is that AFM can visualize non-conductive materials in a non-vacuous (i.e., air or liquid) environment. AFM images of the plasmid DNA are comparable to those by transmission electron microscopy using a rotary shadowing technique, and have the advantage of examining directly the molecule without staining nor coating. The surface structure of human metaphase chromosomes and mouse collagen fibrils demonstrated in air by the non-contact mode AFM is comparable to that obtained by scanning electron microscopy. Quantitative information on the heights of structures is further obtainable from the AFM images. Embedment-free thin tissue-sections are useful for observing intracellular structures by AFM. The present review also shows AFM images of living cultured cells which have been collected in a contact mode in liquid. This technique afforded us three-dimensional observation of the cellular movement with high resolution. Although there are some innate limitations for AFM imaging, the AFM has great potential for providing valuable new information in histology and cytology.
影响因子：
2.9
作者：
BUSTAMANTE, C;VESENKA, J;KELLER, R
通讯作者：
KELLER, R
生物原子力显微镜的最新进展。
DOI：
10.1016/0968-4328(94)00041-n
发表时间：
1995
期刊：
Micron (Oxford, England : 1993)
影响因子：
--
作者：
Yang,J;Shao,Z
通讯作者：
Shao,Z
使用原子力显微镜对活体神经元和神经胶质细胞进行三维成像。
影响因子：
4
作者：
Parpura,V;Haydon,PG;Henderson,E
通讯作者：
Henderson,E