Rho-kinase regulates tissue morphogenesis via non-muscle myosin and LIM-kinase during Drosophila development.
Rho-kinase regulates tissue morphogenesis via non-muscle myosin and LIM-kinase during Drosophila development.
复制标题
Rho-激酶在果蝇发育过程中通过非肌肉肌球蛋白和LIM激酶调节组织形态发生。
DOI：
10.1186/1471-213x-6-38
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发表时间：
2006-08-01
影响因子：
--
通讯作者：
Settleman J
中科院分区：
文献类型：
--
作者：
Verdier V;Guang-Chao-Chen;Settleman J
The Rho-kinases (ROCKs) are major effector targets of the activated Rho GTPase that have been implicated in many of the Rho-mediated effects on cell shape and movement via their ability to affect acto-myosin contractility. The role of ROCKs in cell shape change and motility suggests a potentially important role for Rho-ROCK signaling in tissue morphogenesis during development. Indeed, in Drosophila, a single ROCK ortholog, DRok, has been identified and has been found to be required for establishing planar cell polarity. We have examined a potential role for DRok in additional aspects of tissue morphogenesis using an activated form of the protein in transgenic flies. Our findings demonstrate that DRok activity can influence multiple morphogenetic processes, including eye and wing development. Furthermore, genetic studies reveal that Drok interacts with multiple downstream effectors of the Rho GTPase signaling pathway, including non-muscle myosin heavy chain, adducin, and Diaphanous in those developmental processes. Finally, in overexpression studies, we determined that Drok and Drosophila Lim-kinase interact in the developing nervous system. These findings indicate widespread diverse roles for DRok in tissue morphogenesis during Drosophila development, in which multiple DRok substrates appear to be required.
