Slow muscle myoblasts differentiating in vitro synthesize both slow and fast myosin light chains.
Slow muscle myoblasts differentiating in vitro synthesize both slow and fast myosin light chains.
复制标题
体外分化的慢肌成肌细胞合成慢肌球蛋白轻链和快肌球蛋白轻链。
DOI：
10.1016/0012-1606(81)90438-3
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发表时间：
1981
影响因子：
2.7
通讯作者：
N. Raman
中科院分区：
文献类型：
--
作者：
F. Stockdale;H. Baden;N. Raman
胚胎的快骨骼肌和慢骨骼肌是从共同起源的细胞还是从两个不同的细胞起源发育而来，目前还不清楚。最近的证据表明，在神经支配之前，胚胎的所有肌肉都属于一种类型，即快速型，即，都能快速合成肌球蛋白轻链，但不能缓慢合成。神经支配被认为在快肌向慢肌的转变中起着核心作用。这里报道的实验表明，成肌细胞从慢肌区域的胚胎分离时，在组织培养分化成肌管，合成快速和慢速肌球蛋白轻链，神经支配是不需要启动慢肌球蛋白轻链的合成。
Whether fast and slow skeletal muscles of the embryo develop from cells of a common origin or from two separate cellular origins is not known. Recent evidence suggests that prior to innervation all muscles of the embryo are of one type, the fast type, i.e., all synthesize fast but not slow myosin light chains. Innervation has been thought to play the central role in the shift of a fast to a slow muscle. Experiments reported here demonstrate that myoblasts from slow muscle regions of the embryo when isolated in tissue culture differentiate into myotubes which synthesize both fast and slow myosin light chains, and that innervation is not required to initiate slow myosin light-chain synthesis.