Pigment dispersing factor-dependent and -independent circadian locomotor behavioral rhythms
Pigment dispersing factor-dependent and -independent circadian locomotor behavioral rhythms
复制标题
DOI：
10.1523/jneurosci.4087-07.2008
复制
发表时间：
2008-01-02
影响因子：
5.3
通讯作者：
Holmes, Todd C.
中科院分区：
文献类型：
--
作者：
Sheeba, Vasu;Sharma, Vijay K.;Holmes, Todd C.
昼夜节律起搏器电路由神经元群组成，每个神经元都表达分子振荡，但多个振荡器在电路范围内的协调如何调节有节奏的生理和行为输出仍然是一个悬而未决的问题。为了研究果蝇昼夜节律起搏电路中振荡器相位模式与运动活动节律之间的关系，我们干扰了外侧腹侧神经元（LNv）的电活动和色素分散因子（PDF）水平，并分析了它们对电路不同部分分子振荡和运动活动行为的组合影响。在大约60%的果蝇中，表达pdf的LNv的电活动改变导致最初的行为性心律失常，随后逐渐出现两个同时出现的短周期和长周期昼夜节律行为活动。电路范围内分子振荡的初始非同步性随后出现了一种新的周期（PER）同步模式，即两个背神经元亚群（DN1和DN2）在两个活动回合中出现PER振荡峰值，而其他神经元亚群在两个活动回合中出现单个PER峰值。这种新颖的全电路振荡器同步模式的出现并不伴随着LNv电活动的同步变化。在没有pdf的果蝇中，LNv电活动的改变仅驱动短周期的昼夜活动，这表明不依赖pdf的因素是短周期昼夜活动成分的基础，而长周期昼夜活动成分依赖于pdf。因此，在电操纵的果蝇中，多节奏行为模式是由分子振荡和LNv电活动的全电路协调通过pdf依赖和不依赖的因素来调节的。
Circadian pacemaker circuits consist of ensembles of neurons, each expressing molecular oscillations, but how circuit-wide coordination of multiple oscillators regulates rhythmic physiological and behavioral outputs remains an open question. To investigate the relationship between the pattern of oscillator phase throughout the circadian pacemaker circuit and locomotor activity rhythms in Drosophila, we perturbed the electrical activity and pigment dispersing factor (PDF) levels of the lateral ventral neurons (LNv) and assayed their combinatorial effect on molecular oscillations in different parts of the circuit and on locomotor activity behavior. Altered electrical activity of PDF-expressing LNv causes initial behavioral arrhythmicity followed by gradual long-term emergence of two concurrent short-and long-period circadian behavioral activity bouts in similar to 60% of flies. Initial desynchrony of circuit-wide molecular oscillations is followed by the emergence of a novel pattern of period (PER) synchrony whereby two subgroups of dorsal neurons (DN1 and DN2) exhibit PER oscillation peaks coinciding with two activity bouts, whereas other neuronal subgroups exhibit a single PER peak coinciding with one of the two activity bouts. The emergence of this novel pattern of circuit-wide oscillator synchrony is not accompanied by concurrent change in the electrical activity of the LNv. In PDF-null flies, altered electrical activity of LNv drives a short-period circadian activity bout only, indicating that PDF-independent factors underlie the short-period circadian activity component and that the long-period circadian component is PDF-dependent. Thus, polyrhythmic behavioral patterns in electrically manipulated flies are regulated by circuit-wide coordination of molecular oscillations and electrical activity of LNv via PDF-dependent and -independent factors.