Biochemical characterization of the epithelial dystroglycan complex
Biochemical characterization of the epithelial dystroglycan complex
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DOI：
10.1074/jbc.274.37.26609
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发表时间：
1999-09-10
影响因子：
4.8
通讯作者：
Campbell, KP
中科院分区：
文献类型：
--
作者：
Durbeej, M;Campbell, KP
肌营养不良聚糖是一种广泛表达的细胞外基质受体，在基底膜形成、上皮发育和突触发生中发挥关键作用。肌营养不良聚糖最初在骨骼肌中被定性为肌营养不良蛋白糖蛋白复合物的组成部分，这对肌肉细胞的活力至关重要。尽管骨骼肌中的肌营养不良聚糖复合物已得到很好的表征，但有关骨骼肌外肌营养不良聚糖复合物的结构组成的信息很少。在这里，我们对肺和肾中的肌营养不良聚糖复合物进行了生化表征。我们证明，肺中肌聚糖和肌跨的存在反映了与平滑肌中肌营养不良聚糖的关联。肺中的平滑肌肌营养不良蛋白复合物由肌营养不良蛋白、肌营养不良蛋白/肌营养蛋白、β-、δ-组成，是肌聚糖和肌跨的一个元素，可以通过生化方法与上皮肌营养不良聚糖分离，其与任何已知的肌聚糖或肌跨不相关。类似地，肾上皮细胞中的肌营养不良聚糖与任何肌聚糖或肌跨均不相关。因此，我们的数据表明，非骨骼肌器官中存在如下不同的肌聚糖复合物：一种来自平滑肌，与肌聚糖相关，形成与骨骼肌中类似的复合物，另一种来自上皮细胞。
Dystroglycan is a widely expressed extracellular matrix receptor that plays a critical role in basement membrane formation, epithelial development, and synaptogenesis. Dystroglycan was originally characterized in skeletal muscle as an integral component of the dystrophin glycoprotein complex, which is critical for muscle cell viability. Although the dystroglycan complex has been well characterized in skeletal muscle, there is little information on the structural composition of the dystroglycan complex outside skeletal muscle. Here we have biochemically characterized the dystroglycan complex in lung and kidney. We demonstrate that the presence of sarcoglycans and sarcospan in lung reflects association with dystroglycan in the smooth muscle. The smooth muscle dystroglycan complex in lung, composed of dystroglycan, dystrophin/utrophin, beta-, delta-, is an element of-sarcoglycan, and sarcospan, can be biochemically separated from epithelial dystroglycan, which is not associated with any of the known sarcoglycans or sarcospan. Similarly, dystroglycan in kidney epithelial cells is not associated with any of the sarcoglycans or sarcospan. Thus, our data demonstrate that there are distinct dystroglycan complexes in non-skeletal muscle organs as follows: one from smooth muscle, which is associated with sarcoglycans forming a similar complex as in skeletal muscle, and one from epithelial cells.