Biogenesis of the apical endosome-lysosome complex during differentiation of absorptive epithelial cells in rat ileum.
Biogenesis of the apical endosome-lysosome complex during differentiation of absorptive epithelial cells in rat ileum.
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发表时间：
1991-09
影响因子：
4
通讯作者：
J. Wilson;J. Whitney;M. Neutra
中科院分区：
文献类型：
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作者：
J. Wilson;J. Whitney;M. Neutra
新生大鼠回肠的吸收细胞具有复杂的顶端内吞复合体，由管状和囊泡内体、多囊泡体（MVB）和巨大的溶酶体液泡组成。该系统在妊娠最后 3 天（20-22 天）内快速发育。我们使用微绒毛、内体小管和溶酶体膜的抗原标记物通过超微结构分析和免疫细胞化学来跟踪该复合物的组装。妊娠 19 天时，微绒毛上出现低水平的乳糖酶，但不存在特化的顶内体小管和溶酶体。第20天时，微绒毛乳糖酶的表达增加，内体标记物entubin出现，同时出现特化的顶内体小管。顶端质膜域分化后，顶端内体-溶酶体系统的区室依次组装；首先是内体小管和囊泡，然后是 MVB，并在出生后不久以巨型溶酶体的组装结束。在组装过程的早期阶段，管状内体和溶酶体的膜成分出现在顶端质膜中，然后被限制在各自的细胞内区室中。
Absorptive cells of the neonatal rat ileum have an elaborate apical endocytic complex consisting of tubular and vesicular endosomes, multivesicular bodies (MVB), and a giant lysosomal vacuole. This system develops rapidly over the last 3 days (20-22) of gestation. We followed the assembly of this complex by ultrastructural analysis and immunocytochemistry using antigenic markers for microvilli, endosomal tubules and lysosomal membranes. At 19 days gestation, low levels of lactase appeared on microvilli but specialized apical endosomal tubules and lysosomes were absent. At 20 days, expression of microvillar lactase increased and the endosomal marker entubin appeared, in parallel with the appearance of specialized apical endosomal tubules. The compartments of the apical endosome-lysosome system were assembled sequentially after differentiation of the apical plasma membrane domains; first endosomal tubules and vesicles, followed by MVB, and ending with the assembly of the giant lysosome shortly after birth. During early stages of the assembly process, membrane components of the tubular endosomes and lysosomes appeared in the apical plasma membrane before being restricted to their respective intracellular compartments.