GmUGT73F4 plays important roles in enhancing seed vitality and tolerance to abiotic stresses in transgenic Arabidopsis
GmUGT73F4 plays important roles in enhancing seed vitality and tolerance to abiotic stresses in transgenic Arabidopsis
复制标题
DOI：
10.1007/s11240-022-02270-z
复制
发表时间：
2022-03-26
影响因子：
3
通讯作者：
Ma, Hao
中科院分区：
文献类型：
--
作者：
Hu, Huimin;Qian, Peipei;Ma, Hao
UDP-糖基转移酶（UGT）具有共同的GTB（糖基转移酶_GTB型超家族拓扑结构），通常参与修饰小分子并控制植物生长发育过程中的许多代谢过程。然而，GmUGT 73 F4在大豆中的功能报道较少。在这项研究中，GmUGT 73 F4被分离和表征。该基因编码476个氨基酸残基，分子量为53.2kDa，等电点为6.89，在成熟豆荚和发育中的种子中表达量较高。其编码蛋白定位于大豆细胞质膜和细胞核中。高温高湿对GmUGT 73 F4基因表达有显著的诱导作用。GmUGT 73 F4基因在拟南芥中的过表达增强了对HTH胁迫的耐受性，提高了种子活力。GmUGT 73 F4与金属硫蛋白4（MT 4）相互作用，可能通过上调胁迫相关基因表达和清除活性氧（ROS）来响应盐、干旱和低温胁迫以及阿坝诱导。简言之，我们的研究结果表明GmUGT 73 F4在增强植物种子活力和对非生物胁迫的耐受性方面起着重要作用。Key message本研究发现GmUGT 73 F4在拟南芥中的过表达可以提高种子活力和对非生物胁迫的耐受性。
UDP-Glycosyltransferases (UGTs) share a common GTB (Glycosyltransferase_GTB-type Superfamily topology), typically participating in modifying small molecules and controlling many metabolic processes during plant growth and development. However, GmUGT73F4 in soybean is less reported about its functions. In this study, GmUGT73F4 was isolated and characterized. It encoded a peptide of 476 amino acids residues with a calculated molecular mass of 53.2 kDa and a pI of 6.89 and was highly expressed in mature pods and developing seeds. Its encoding protein was located in cell plasma membrane and nucleus in soybean. GmUGT73F4 was induced dramatically in expression level by high temperature and humidity (HTH). The overexpression of GmUGT73F4 in Arabidopsis enhanced tolerance to HTH stress and promoted seed vitality. GmUGT73F4 was found to be able to interact with metallothionein 4 (MT4), responding to salt, drought, and cold stresses as well as ABA inducement maybe through up-regulation of the stress-related genes and scavenging of reactive oxygen species (ROS). Briefly, our findings demonstrated that GmUGT73F4 plays important roles in enhancing seed vitality and tolerance to abiotic stresses in plants.Key message This study found that overexpression of GmUGT73F4 in Arabidopsis could promote seed vigor and tolerance to abiotic stresses.