Basal forebrain neurons and memory: a biochemical, histological, and behavioral study of differential vulnerability to ibotenate and quisqualate.
Basal forebrain neurons and memory: a biochemical, histological, and behavioral study of differential vulnerability to ibotenate and quisqualate.
复制标题
基底前脑神经元和记忆：对鹅膏酯和使君子酯的不同易感性的生化、组织学和行为研究。
DOI：
10.1037//0735-7044.106.6.909
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发表时间：
1992
影响因子：
1.9
通讯作者：
Walker,LC
中科院分区：
文献类型：
--
作者：
Wenk,GL;Harrington,CA;Tucker,DA;Rance,NE;Walker,LC
在大鼠中研究了基底前脑细胞对鹅膏酯（IBO）或使君子酸（QUIS）的不同脆弱性。 IBO 还与胱氨酸 (CYS) 或锌 (Zn) 共同注射。结合神经降压素、P 物质和生长抑素的放射免疫测定，对皮质胆碱乙酰转移酶 (ChAT) 和谷氨酸脱羧酶 (GAD) 活性、神经降压素受体和高亲和力胆碱摄取位点进行定量；神经降压素、生长抑素、亮氨酸脑啡肽和 ChAT 阳性细胞的免疫细胞化学；以及生长抑素、P 物质和脑啡肽 mRNA 的原位杂交组织化学。与对照组的表现相比，IBO+Zn或IBO+CYS大鼠在T迷宫中的连续交替表现优于IBO大鼠，而QUIS大鼠的表现未受影响。在这些被检查的神经递质系统中，只有 ChAT 免疫反应细胞容易受到 IBO 或 QUIS 的影响。然而，胆碱能细胞损失与表现受损并不相关。
The differential vulnerability of basal forebrain cells to ibotenate (IBO) or quisqualate (QUIS) was investigated in rats. IBO was also coinjected with cystine (CYS) or zinc (Zn). Cortical choline acetyltransferase (ChAT) and glutamate decarboxylase (GAD) activity, neurotensin receptors, and high-affinity choline uptake sites were quantified in conjunction with radioimmunoassays for neurotensin, substance P, and somatostatin; immunocytochemistry for neurotensin-, somatostatin-, Leu-enkephalin-, and ChAT-positive cells; and in situ hybridization histochemistry of somatostatin, substance P, and enkephalin mRNAs. Compared with the performance of controls, continuous alternation performance in a T maze of IBO+ Zn or IBO+ CYS rats was better than that of IBO rats, whereas the performance of QUIS rats was unimpaired. Of those neurotransmitter systems examined, only ChAT-immunoreactive cells were vulnerable to IBO or QUIS. However, cholinergic cell loss did not correlate with impaired performance.