HUMAN EOSINOPHIL MAJOR BASIC-PROTEIN CAUSES HYPERREACTIVITY OF RESPIRATORY SMOOTH-MUSCLE - ROLE OF THE EPITHELIUM
HUMAN EOSINOPHIL MAJOR BASIC-PROTEIN CAUSES HYPERREACTIVITY OF RESPIRATORY SMOOTH-MUSCLE - ROLE OF THE EPITHELIUM
复制标题
DOI：
10.1164/ajrccm/138.3.685
复制
发表时间：
1988-09-01
期刊：
影响因子：
--
通讯作者：
VANHOUTTE, PM
中科院分区：
文献类型：
--
作者：
FLAVAHAN, NA;SLIFMAN, NR;VANHOUTTE, PM
主要碱性蛋白（MBP）是与嗜酸性粒细胞颗粒相关的几种阳离子蛋白之一，对呼吸道上皮细胞具有细胞毒性，存在于哮喘患者的痰液中以及死于哮喘的患者受损的支气管上皮细胞上。进行本实验是为了确定MBP对气道平滑肌反应性的影响。将豚鼠气管环（其中一些气管环的上皮细胞已通过擦拭轻轻去除）在含有缓冲液或人MBP（100μg/ml）的改良Krebs - Ringer碳酸氢盐溶液中孵育5小时。然后将气管环悬挂在装有含吲哚美辛的Krebs - Ringer溶液的器官腔室中，用于等长张力记录。MBP不影响上皮已被去除的气管环的反应性，但显著增强未擦拭气管环对乙酰胆碱和组胺的反应性。结果表明，MBP通过抑制上皮细胞而非平滑肌细胞的功能，导致呼吸道平滑肌高反应性，这可能是慢性呼吸道疾病中观察到的高反应性的原因之一。
Major basic protein (MBP), one of several cationic proteins associated with the eosinophil granule, is cytotoxic to respiratory epithelium and is present in the sputum of asthmatics and on damaged bronchial epithelium from patients dying of asthma. The present experiments were performed in order to determine the effects of MBP on the responsiveness of airway smooth muscle. Rings of guinea pig trachea, in some of which the epithelium had been gently removed by rubbing, were incubated for 5 h in modified Krebs-Ringer bicarbonate solution containing buffer or human MBP (100 .mu.g/ml). The tracheal rings were then suspended for isometric tension recording in organ chambers filled with Krebs-Ringer solution containing indomethacin. MBP did not affect the reactivity of rings in which the epithelium had been removed, but significantly augmented that of unrubbed rings to acetylcholine and histamine. The results suggest that MBP, by inhibiting the function of epithelial but not smooth muscle cells, causes hyperreactivity of respiratory smooth muscle, which may contribute to the hyperreactivity observed in chronic respiratory disease.