Graphene oxide-coated porous titanium for pulp sealing: an antibacterial and dentino-inductive restorative material
Graphene oxide-coated porous titanium for pulp sealing: an antibacterial and dentino-inductive restorative material
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用于牙髓封闭的氧化石墨烯涂层多孔钛：一种抗菌和牙本质诱导的修复材料
DOI：
10.1039/d0tb00697a
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发表时间：
2020-07-14
影响因子：
7
通讯作者：
Jiang, Xinquan
中科院分区：
文献类型：
--
作者：
Sun, Ningjia;Yin, Shi;Jiang, Xinquan
牙髓盖盖、截髓、再生等牙髓治疗技术都是基于保留活髓的原则。然而，目前还没有开发出能够同时保护牙髓活力和修复咬合形态的特殊牙体修复材料。传统的盖髓材料由于其长期溶解性和力学性能差，不能作为牙体修复材料。钛具有良好的生物相容性、可加工性和力学性能，被认为是一种很有前途的牙髓密封材料，在牙科中有着广泛的应用。最初，我们提出了“牙体整合”的概念，它代表牙髓与牙髓密封材料表面之间直接的牙本质状矿化接触；在此，我们报道了一种新型抗菌牙本质感应材料的制备，通过微弧氧化（MAO），结合自组装氧化石墨烯（GO）进行钛表面改性。氧化石墨烯涂层的分层微孔/纳米孔结构为人牙髓干细胞的成牙分化提供了合适的微环境，氧化石墨烯的负载有助于抗菌活性。采用扫描电子显微镜（SEM）、能量色散x射线能谱和拉曼光谱进行结构和元素分析。体外实验包括细胞粘附、活/死和CCK-8测定、碱性磷酸酶活性和钙沉积测定、实时聚合酶链反应、western blot分析和免疫荧光染色来检测细胞粘附、活力、增殖、矿化和成牙分化能力。采用扫描电镜（SEM）、平板平板（spread plate）、活/死试验（Live/Dead）和Alamar蓝试验（Alamar blue）分析对变形链球菌的抗菌性能。Ti-MAO-1.0 mg mL（-1）氧化石墨烯组表现出良好的细胞粘附、成牙细胞分化、矿化和抗菌能力，有利于牙体整合。
Pulp treatment techniques such as pulp capping, pulpotomy and pulp regeneration are all based on the principle of preserving vital pulp. However, specific dental restorative materials that can simultaneously protect pulp vitality and repair occlusal morphology have not been developed thus far. Traditional pulp capping materials cannot be used as dental restorative materials due to their long-term solubility and poor mechanical behavior. Titanium (Ti) is used extensively in dentistry and is regarded as a promising material for pulp sealing because of its favorable biocompatibility, processability and mechanical properties. Originally, we proposed the concept of "odontointegration", which represents direct dentin-like mineralization contact between pulp and the surface of the pulp sealing material; herein, we report the fabrication of a novel antibacterial and dentino-inductive material via micro-arc oxidation (MAO), incorporating self-assembled graphene oxide (GO) for Ti surface modification. The hierarchical micro/nanoporous structure of the MAO coating provides a suitable microenvironment for odontogenic differentiation of human dental pulp stem cells, and GO loading contributes to antibacterial activity. Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy and Raman spectroscopy were employed for structure and elemental analysis. In vitro studies, including cell adhesion, Live/Dead and CCK-8 assays, alkaline phosphatase activity and calcium deposition assay, real-time polymerase chain reaction, western blot analysis and immunofluorescence staining were used to examine cell adhesion, viability, proliferation, mineralization, and odontogenic differentiation ability. Antibacterial properties against Streptococcus mutans were analyzed by SEM, spread plate, Live/Dead and Alamar blue tests. The Ti-MAO-1.0 mg mL(-1) GO group exhibited excellent cell adhesion, odontoblast differentiation, mineralization, and antibacterial ability, which are beneficial to odontointegration.