Structural difference between polymerized and non-polymerized fragment X, obtained by plasmin digest of fibrinogen.
Structural difference between polymerized and non-polymerized fragment X, obtained by plasmin digest of fibrinogen.
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通过纤溶酶消化纤维蛋白原获得的聚合和非聚合片段 X 之间的结构差异。
DOI：
10.1016/0141-8130(93)90048-q
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发表时间：
1993
影响因子：
8.2
通讯作者：
Swadesh,JK
中科院分区：
文献类型：
--
作者：
Sato,H;Swadesh,JK
片段 X (LMrFX) 是从人纤维蛋白原的后期 2 纤溶酶消化物中获得的低分子量制剂。凝血酶处理的LMrFX制剂会导致聚合受损，被进一步细分为聚合和非聚合组分。通过 SDS-PAGE 和免疫化学方法检查级分。在聚合级分中，在还原态的 SDS-PAGE 上观察到的肽带比非聚合级分中更多。两种级分的 Aα、Bβ 和 γ 链中包含相似数量的内部裂解，这些链通过二硫键连接。因此，γ链的羧基末端区域和Bβ链的氨基末端部分的聚合位点的部分缺陷以及内部裂解被认为参与了凝血酶诱导的LMrFX聚合的损害。
Fragment X (LMrFX) was obtained as low molecular weight preparations from a late stage 2 plasmin digest of human fibrinogen. The thrombin-treated LMrFX preparations, which resulted in impaired polymerization, were further subfractionated into polymerized and non-polymerized components. The fractions were examined by SDS-PAGE and immunochemical methods. In polymerized fractions, more peptide bands were observed on SDS-PAGE in the reduced state than in non-polymerized fractions. Both fractions contained a similar number of internal cleavages in the Aα, Bβ and γ chains, which are linked by disulfide bonds. Thus, the partial deficiencies in polymerization sites of the carboxy terminal region of the γ chain and the amino terminal portions of the Bβ chain, as well as internal cleavage, were considered to participate in the impairment of the thrombin-induced polymerization of LMrFX.