A novel reticular stromal structure in lymph node cortex: an immuno-platform for interactions among dendritic cells, T cells and B cells
A novel reticular stromal structure in lymph node cortex: an immuno-platform for interactions among dendritic cells, T cells and B cells
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DOI：
10.1093/intimm/dxh113
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发表时间：
2004-08-01
影响因子：
4.4
通讯作者：
Shimizu, A
中科院分区：
文献类型：
--
作者：
Katakai, T;Hara, T;Shimizu, A
为了有效的适应性免疫，淋巴结（LN）配备了一个战略组织的微架构，这在很大程度上是由网状网络（RN）的支持。RN可以通过荧光免疫组织化学结合使用单克隆抗体EIR-TR 7的共聚焦成像清楚地可视化，并且可以细分为四个结构不同的区域，每个区域与不同免疫细胞亚群的位置密切相关。此外，我们注意到在T区和B区的边界处有一个被称为“皮质脊”的特征性网状结构，树突状细胞优先聚集在其中。冷冻切片的体外粘附试验表明，树突状细胞的皮质嵴，而不是更深的皮质的偏好。连续转移实验还表明，携带抗原的树突状细胞从外周组织迁移到该区域，特别是在高内皮微静脉附近，并锚定在网状纤维上，等待与抗原特异性T细胞相互作用。总之，本研究中获得的发现为LN基质网如何作为组织区室化和免疫应答的专门“免疫平台”工作提供了新的见解。
For efficient adaptive immunity, the lymph nodes (LNs) are equipped with a strategically organized microarchitecture, which is largely supported by the reticular network (RN). The RN can be clearly visualized by fluorescence immunohistochemistry coupled with confocal imaging using a monoclonal antibody, EIR-TR7, and can be subdivided into four structurally distinct regions, each of which correlates well with the location of distinct immune cell subsets. In addition, we noticed a characteristic reticular structure designated the 'cortical ridge' at the boundary of the T and B zone, in which dendritic cells are preferentially accumulated. In vitro adhesion assays of frozen sections demonstrated a preference of dendritic cells for the cortical ridge rather than the deeper cortex. Adoptive transfer experiments also demonstrated that antigen-bearing dendritic cells migrated to this region from peripheral tissues, especially in the vicinity of the high endothelial venules, and were anchored on the reticular fibers waiting to interact with the antigen-specific T cells. Taken together, the findings obtained in this study provide new insights into how the LN stromal reticulum works as a specialized 'immuno-platform' for tissue compartmentalization and the immune response.