A novel lineage of sulfate-reducing microorganisms:: Thermodesulfobiaceae fam. nov., Thermodesulfobium narugense, gen. nov., sp nov., a new thermophilic isolate from a hot spring
A novel lineage of sulfate-reducing microorganisms:: Thermodesulfobiaceae fam. nov., Thermodesulfobium narugense, gen. nov., sp nov., a new thermophilic isolate from a hot spring
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DOI：
10.1007/s00792-003-0320-0
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发表时间：
2003-08-01
期刊：
影响因子：
2.9
通讯作者：
Hanada, S
中科院分区：
文献类型：
--
作者：
Mori, K;Kim, H;Hanada, S
从日本鸣湖的温泉中分离到一株以Na82(T)为代表的新型硫酸盐还原微生物。该菌株是一株中等嗜热的自养细菌，能通过硫酸盐呼吸在H-2/CO2上生长。该菌株能在硝酸盐替代硫酸盐的条件下生长，呼吸醌类物质为孟喹酮-7和孟喹酮-7(H-2)。16S rRNA基因序列的系统发育分析表明，菌株Na82(T)是该区域细菌的成员，与任何已知细菌以及其他硫酸盐还原细菌(序列相似性小于80%)都有距离。DsrAB基因(异化亚硫酸盐还原酶的α和β亚基)序列的系统发育分析也表明，菌株Na82(T)与其他硫酸盐还原菌的亲缘关系不密切。根据表型和系统发育数据，建立了该菌株的新分类群。我们建议将其命名为纳鲁金热脱氢杆菌基因。Nov.，sp.11月以菌株Na82(T)(=DSM 14796(T)=JCM 11510(T))为模式菌株。此外，还发现了一个新的科--热脱氧菌科。Nov.，为该属提出。
A novel type of a sulfate-reducing microorganism, represented by strain Na82(T), was isolated from a hot spring in Narugo, Japan. The isolate was a moderate thermophilic autotroph that was able to grow on H-2/CO2 by sulfate respiration. The isolate could grow with nitrate in place of sulfate, and possessed menaquinone-7 and menaquinone-7(H-2) as respiratory quinones. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Na82(T) was a member of the domain Bacteria and distant from any known bacteria, as well as from other sulfate-reducing bacteria (sequence similarities less than 80%). The phylogenetic analysis of the dsrAB gene (alpha and beta subunits of dissimilatory sulfite reductase) sequence also suggested that strain Na82(T) was not closely related to other sulfate reducers. On the basis of the phenotypic and phylogenetic data, a new taxon is established for the isolate. We proposed the name Thermodesulfobium narugense gen. nov., sp. nov. with strain Na82(T) (=DSM 14796(T)=JCM 11510(T)) as the type strain. Furthermore, a new family, Thermodesulfobiaceae fam. nov., is proposed for the genus.