Transcriptomics approach to investigate zebrafish heart regeneration
Transcriptomics approach to investigate zebrafish heart regeneration
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DOI：
10.2459/jcm.0b013e3283375900
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Izpisua Belmonte, Juan Carlos
中科院分区：
文献类型：
--
作者：
Sleep, Eduard;Boue, Stephanie;Izpisua Belmonte, Juan Carlos
在哺乳动物中，在心肌梗塞发作后，受损的心肌被瘢痕组织取代，心肌细胞增殖可忽略不计。相比之下，斑马鱼显示出广泛的再生能力，因为它们能够在部分心室切除后完全恢复失去的心脏组织。虽然关于驱动再生反应的早期信号以及每种心脏细胞类型在这一过程中的相对作用的问题仍然需要回答，但斑马鱼正在成为一种非常有价值的工具，以了解心脏再生并制定可能对治疗人类心脏病具有潜在价值的策略。在这里，我们进行了全基因组转录谱分析，重点是斑马鱼心脏再生的早期时间点，并将我们的结果与先前发表的数据进行了比较。我们的分析证实了几个转录本的差异表达，并确定了其他基因的表达差异调节斑马鱼心脏再生。我们通过常规和/或定量逆转录聚合酶链反应（RT-PCR）验证了微阵列数据。对于这些基因的一个子集，其表达模式进行了原位杂交分析，并显示在心脏的再生区域上调。我们的研究结果为斑马鱼心脏再生的生物学提供了新的见解，加上未来在哺乳动物中的实验，可能对临床应用具有潜在的意义。J.B. Vasc Med 11：369-380（C）2010意大利心脏病学联合会。
In mammals, after a myocardial infarction episode, the damaged myocardium is replaced by scar tissue with negligible cardiomyocyte proliferation. Zebrafish, in contrast, display an extensive regenerative capacity, as they are able to restore completely lost cardiac tissue after partial ventricular amputation. Although questions about the early signals that drive the regenerative response and the relative role of each cardiac cell type in this process still need to be answered, the zebrafish is emerging as a very valuable tool to understand heart regeneration and to devise strategies that may be of potential value to treat human cardiac disease. Here, we performed a genomewide transcriptome profile analysis focusing on the early time points of zebrafish heart regeneration and compared our results with those of previously published data. Our analyses confirmed the differential expression of several transcripts and identified additional genes whose expression is differentially regulated during zebrafish heart regeneration. We validated the microarray data by conventional and/or quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). For a subset of these genes, their expression pattern was analyzed by in-situ hybridization and shown to be upregulated in the regenerating area of the heart. Our results offer new insights into the biology of heart regeneration in the zebrafish and, together with future experiments in mammals, may be of potential interest for clinical applications. J Cardiovasc Med 11:369-380 (C) 2010 Italian Federation of Cardiology.