Polyamine concentrations in the brain of vitamin B12-deficient rats
Polyamine concentrations in the brain of vitamin B12-deficient rats
复制标题
DOI：
10.1177/153537020322800913
复制
发表时间：
2003-10-01
影响因子：
3.2
通讯作者：
Mitsuma, T
中科院分区：
文献类型：
--
作者：
Adachi, K;Izumi, M;Mitsuma, T
为了研究维生素B-12缺乏所致神经元变性的病理生理学机制，我们采用高效液相色谱-荧光检测法检测了脑区和肝脏中多胺腐胺、亚精胺和精胺的浓度。雄性Wistar大鼠以正常饲料或维生素B-12缺乏饲料喂养20周。未观察到明显的行为变化。与对照组相比，饲喂维生素B-12缺乏饲料的大鼠血清维生素B-12和肝脏蛋氨酸浓度显著降低，肝脏同型半胱氨酸浓度显著升高。维生素B-12缺乏与肝脏和大脑某些区域的亚精胺、亚精胺浓度降低有关，尽管没有观察到行为异常。这些结果提示维生素B-12缺乏可能通过扰乱大鼠脑内多胺浓度而在神经元变性中起作用。
To study the pathophysiology of the neuronal degeneration in vitamin B-12 deficiency, we investigated the concentrations of the polyamines putrescine, spermidine, and spermine in brain regions and liver using high-performance liquid chromatography with fluorescence detection. Male Wistar rats were fed either a control or vitamin B-12-deficient diet for 20 weeks. No remarkable behavioral changes were observed. Serum vitamin B-12 and hepatic methionine concentrations were significantly lower and hepatic homocysteine was elevated in rats fed vitamin B-12-deficient diet than in controls. Vitamin B-12 deficiency was associated with decreased concentrations of spermidine, spermidine in liver and some regions of brain, although there were no observed abnormalities in behavior. These results suggest that vitamin B-12 deficiency may play a role in neuronal degeneration through the disturbance of polyamine concentrations in rat brain.