Paenibacillus polymyxa SQR-21 systemically affects root exudates of watermelon to decrease the conidial germination of Fusarium oxysporum f.sp niveum
Paenibacillus polymyxa SQR-21 systemically affects root exudates of watermelon to decrease the conidial germination of Fusarium oxysporum f.sp niveum
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多粘类芽孢杆菌 SQR-21 系统性地影响西瓜的根部分泌物，以减少尖镰孢分生孢子的萌发。
DOI：
10.1007/s11104-010-0660-3
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发表时间：
2011-04-01
期刊：
影响因子：
4.9
通讯作者：
Shen, Qirong
中科院分区：
文献类型：
--
作者：
Ling, Ning;Huang, Qiwei;Shen, Qirong
多粘类芽孢杆菌（Paenibacillus polymyxa）SQR-21是一种具有生物防治西瓜枯萎病潜力的细菌。研究了西瓜根系分泌物对FON分生孢子萌发的影响。与对照相比，SQR-21接种植株的根分泌物降低了分生孢子的萌发率，而FON接种植株的根分泌物促进了分生孢子的萌发。接种30 d后，根分泌物的萌发率最高，是对照的1.35倍。设计了一个分根系统，以验证SQR-21接种或FON接种西瓜根系分泌模式的改变不仅是局部的，而且是系统的。西瓜根系分泌物中含有肉桂酸。肉桂酸对FON分生孢子萌发的影响试验表明，肉桂酸在0 ~ 30 μg/ml浓度范围内对FON分生孢子萌发均有促进作用。总之，SQR-21和FON都系统地影响西瓜根系分泌物。这些结果有助于更好地了解植物-微生物间的通讯，并为西瓜枯萎病的生物防治提供指导。
Paenibacillus polymyxa SQR-21 has been identified as a potential agent for the biocontrol of Fusarium wilt in watermelon, which is caused by the pathogenic fungus Fusarium oxysporum f.sp. niveum (FON). In the present study, the effects of root exudates from watermelon plants inoculated or non-inoculated with either SQR-21 or FON on conidial germination of FON were investigated. Compared to the control, conidial germination was decreased with root exudates from SQR-21-inoculated plants, but conidial germination was enhanced by root exudates from FON-inoculated plants. Maximal germination was found with root exudates from FON-inoculated plants after 30 d, which was 1.35 times more germination than the control. A split-root system was designed to verify that the alterations of the exudation pattern in SQR-21- inoculated or FON-inoculated watermelon roots were not only local, but also systemic. Cinnamic acid was found in the watermelon root exudates. An assay to test the effects of cinnamic acid on conidial germination of FON revealed that the stimulation of conidial germination was observed from cinnamic acid concentrations ranging from 0 to 30 μg/ml. In conclusion, both of SQR-21 and FON systemically affects watermelon root exudates. These results will help to the better understanding of the plant-microbe communication and will guide to improve the biocontrol strategies against Fusarium wilt of watermelon plants.