The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings
The vasorelaxing effect of hydrogen sulfide on isolated rat aortic rings versus pulmonary artery rings
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硫化氢对离体大鼠主动脉环与肺动脉环的血管舒张作用
DOI：
10.1038/aps.2011.9
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发表时间：
2011-04-01
影响因子：
8.2
通讯作者：
Du, Jun-bao
中科院分区：
文献类型：
--
作者：
Sun, Yan;Tang, Chao-shu;Du, Jun-bao
目的：比较硫化氢（H2 S）对离体大鼠主动脉和肺动脉环的舒张作用，并探讨其作用机制。胱硫醚γ裂解酶（CSE）、胱硫醚β合成酶（CBS）、3-巯基丙酮酸硫转移酶（3 MST）、SUR 2B和Kir 6的表达。结果：NaHS可引起大鼠主动脉和肺动脉环舒张，并呈剂量依赖性。NaHS使主动脉环更大程度扩张（50、100、200、500和1000 μmol/L浓度组分别为16.4%、38.4%、64.1%、84.3%和95.9%）（50、100、200、500和1000 μmol/L浓度下分别为10.1%、22.2%、50.6%、73.6%和84.6%）。对主动脉环血管舒张作用的EC 50为152.17 μmol/L，对肺动脉环血管舒张作用的EC 50为233.65 μmol/L。细胞膜和线粒体膜KATP通道阻断剂均能显著阻断H2S的舒张作用（P< 0.01）。相反，只有细胞膜KATP通道阻断剂抑制H2S诱导的肺动脉环血管舒张。SUR 2B mRNA和蛋白在主动脉环中的表达高于肺动脉环。胱硫醚γ-裂解酶（CSE），而不是胱硫醚β合成酶（CBS）的表达在主动脉环高于肺动脉环。3-结论：特定浓度H2 S对离体主动脉环的舒张作用比对肺动脉环的作用更明显，其机制可能与KATP通道亚基SUR 2 B表达增加有关。
Aim:To compare the vasorelaxing effects of hydrogen sulfide (H 2 S) on isolated aortic and pulmonary artery rings and to determine their action mechanisms.Methods:H 2 S-induced vasorelaxation of isolated rat aortic versus pulmonary artery rings under 95% O 2 and 5% CO 2 was analyzed. The expression of cystathinonine gamma-lyase (CSE), cystathionine beta synthase (CBS), 3-mercaptopyruvate sulfurtransferase (3MST), SUR2B and Kir6. 1 was examined.Results:NaHS caused vasorelaxation of rat aortic and pulmonary artery rings in a dose-dependent manner. NaHS dilated aortic rings to a greater extent (16.4%, 38.4%, 64.1%, 84.3%, and 95.9% at concentrations of 50, 100, 200, 500, and 1000 μmol/L, respectively) than pulmonary artery rings (10.1%, 22.2%, 50.6%, 73.6%, and 84.6% at concentrations of 50, 100, 200, 500 and 1000 μmol/L, respectively). The EC 50 of the vasorelaxant effect for aortic rings was 152.17 μmol/L, whereas the EC 50 for pulmonary artery rings was 233.65 μmol/L. The vasorelaxing effect of H 2 S was markedly blocked by cellular and mitochondrial membrane K ATP channel blockers in aortic rings (P< 0.01). In contrast, only the cellular membrane K ATP channel blocker inhibited H 2 S-induced vasorelaxation in pulmonary artery rings. SUR2B mRNA and protein expression was higher in aortic rings than in pulmonary artery rings. Cystathinonine gamma-lyase (CSE) but not cystathionine beta synthase (CBS) expression in aortic rings was higher than in pulmonary artery rings. 3-Mercapto pyruvate sulfurtransferase (3MST) mRNA was lower in aortic rings than in pulmonary artery rings.Conclusion:The vasorelaxing effect of H 2 S on isolated aortic rings was more pronounced than the effect on pulmonary artery rings at specific concentrations, which might be associated with increased expression of the K ATP channel subunit SUR2B.