Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection
Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection
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DOI：
10.1007/s00705-002-0838-y
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发表时间：
2002-08-01
影响因子：
2.7
通讯作者：
Aziz, AJ
中科院分区：
文献类型：
--
作者：
Kono, Y;Yusnita, Y;Aziz, AJ
在 Vero 细胞培养物中，从疑似尼帕病毒感染的猪的肺部分离出一种名为 Oya 病毒的病毒。该病毒呈球形，有包膜RNA病毒，直径79 nm。为了鉴定 Oya 病毒，进行了 RT-PCR。 Bunyavirus 属 Simbu 血清群的 S-RNA 通用引物组能够从 Oya 病毒 RNA 中扩增 cDNA。产品序列数据显示，Oya病毒S-RNA片段部分基因与已发表的Simbu血清群病毒cDNA序列有65-70%的同源性。数据的系统发育分析表明，Oya病毒属于Simbu血清群，但在基因上与分子分析的血清群病毒不同。血清学调查显示，该病毒在马来西亚分布广泛且密集。
A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the genus Bunyavirus was able to amplify a cDNA from Oya virus RNA. The sequence data of the product revealed that the partial gene of Oya virus S-RNA segment had 65-70% homology with published cDNA sequences of Simbu serogroup viruses. The phylogenetic analysis of the data showed that the Oya virus is grouped in Simbu serogroup, but is genetically distinct from the serogroup viruses that have been analyzed molecularly. Serological surveys revealed that the virus distributed widely and densely in Malaysia.