Carbon and nitrogen stable isotopic offsets between diet and hair/feces in captive orangutans.
Carbon and nitrogen stable isotopic offsets between diet and hair/feces in captive orangutans.
复制标题
圈养猩猩的饮食和毛发/粪便之间的碳和氮稳定同位素抵消。
DOI：
10.1007/s10329-021-00940-8
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发表时间：
2021
期刊：
影响因子：
1.7
通讯作者：
Kuze N
中科院分区：
文献类型：
--
作者：
Tsutaya T;Ogawa NO;Nomura T;Shimizu M;Ohkouchi N;Kuze N
估计稳定同位素补偿值是至关重要的饮食重建。虽然对野生非人灵长类动物稳定同位素生态学的研究总体上正在增加，但只有少数研究涉及实验室实验。本研究首次报道了红毛猩猩毛发和粪便中碳、氮稳定同位素偏移值。在持续1周的实验中，记录了6只圈养婆罗洲猩猩（Pongo pygmaeus）个体的每种食物的重量。采集食物、毛发和粪便样品几天，并使用元素分析仪/同位素比质谱仪测量其稳定的碳和氮同位素比。在保存过程中，用乙醇处理粪便子样品。MonteCarlo分析表明，毛发和饲料之间碳和氮补偿值的95%置信区间（CI）分别为+0.9‰ ~+3.9‰和+2.3‰ ~+4.5‰。粪便和饲料之间碳和氮补偿值的95%CI分别为-3.7 ‰至-0.9 ‰和+0.3‰至+2.7‰。乙醇处理对粪便稳定同位素比值的影响尚不清楚，也不确定。计算的偏移值圈养猩猩的头发是类似于在其他非人类灵长类动物的报告，虽然那些粪便表现出更大的种间差异。在这项研究中估计的偏移值有助于同位素研究到自由放养的猩猩谁是在大多数野生环境中极度濒危的喂养生态。
Estimating stable isotopic offset values is crucial for dietary reconstructions. Although research into stable isotope ecology of wild nonhuman primates is increasing overall, only a minority of studies involve laboratory experiments. This study is the first to report the carbon and nitrogen stable isotopic offset values in hair and feces of orangutans. During an experiment lasting 1 week, the weight of each consumed food item was recorded for each of six captive Bornean orangutan (Pongo pygmaeus) individuals. The food, hair, and fecal samples were collected for a few days, and their stable carbon and nitrogen isotope ratios were measured using an elemental analyzer/isotope ratio mass spectrometer. Subsamples of feces were treated with ethanol during the preservation process. Monte Carlo analyses showed that the 95% confidence intervals (CIs) of the carbon and nitrogen offset values between hair and diet were +0.9‰ to +3.9‰ and +2.3‰ to +4.5‰, respectively. The 95% CIs of the carbon and nitrogen offset values between feces and diet were −3.7‰ to −0.9‰ and +0.3‰ to +2.7‰, respectively. The effect of ethanol treatment on the stable isotope ratios of feces was unclear and inconclusive. The computed offset values of hair in captive orangutans are similar to those reported in other nonhuman primates, although those of feces showed greater interspecies variations. The offset values estimated in this study contribute to isotopic studies into the feeding ecology of free-ranging orangutans who are critically endangered in most wild settings.