A new IL-1 receptor inhibitor 139A: Fermentation, isolation, physico-chemical properties and structure
A new IL-1 receptor inhibitor 139A: Fermentation, isolation, physico-chemical properties and structure
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DOI：
10.7164/antibiotics.56.87
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发表时间：
2003-02-01
影响因子：
3.3
通讯作者：
Li, BY
中科院分区：
文献类型：
--
作者：
Chen, J;Wu, JB;Li, BY
已知白细胞介素（IL）-1是一种细胞因子，其在病理状况如脓毒性休克、炎症和自身免疫疾病中起主要作用，因此，降低IL-1活性的方法对临床医学具有影响。抑制IL-1与IL-1受体的结合是方法之一。从链霉菌139的发酵液中分离得到一种新的白细胞介素1受体抑制剂139 A。发酵液滤液经Diaion HP-20、阳离子交换树脂和DEAESephadex柱层析纯化。A-25通过光谱分析确定了139 A为多糖，并通过固定化配体IL-1受体结合试验（IL-1 LRBA）证实了139 A能竞争性抑制IL-1与IL-1受体的结合。
Interleukin (IL)-1 is known to be a cytokine which plays a major role in pathological conditions like septic shock, inflammation and auto-immune disease, hence, methods that reduce the activity of IL-1 have an impact on clinical medicine. Inhibiting the binding of IL-1 to IL-1 receptors is one of the methods. A new inhibitor of IL-1 receptor, 139A, was isolated from the fermentation broth of Streptomyces sp.139. It was extracted from the broth filtrate, purified by Diaion HP-20, cation exchange resin and DEAE Sephadex. A-25. 139A was identified as polysaccharide, its structure was elucidated on the basis of spectral analysis,the immobilized ligand IL-1 receptor binding assay (IL-1LRBA) proved 139A can competitively inhibits the binding of IL-1 to IL-1 receptors.