Immunohistochemical validation of multiple phospho-specific epitopes for estrogen receptor alpha (ERalpha) in tissue microarrays of ERalpha positive human breast carcinomas.
Immunohistochemical validation of multiple phospho-specific epitopes for estrogen receptor alpha (ERalpha) in tissue microarrays of ERalpha positive human breast carcinomas.
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对 ERα 阳性人乳腺癌组织微阵列中雌激素受体 α (ERα) 的多个磷酸化特异性表位进行免疫组织化学验证。
DOI：
10.1007/s10549-008-0267-z
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发表时间：
2009
影响因子：
3.8
通讯作者：
Murphy,LeighC
中科院分区：
文献类型：
--
作者：
Skliris,GeorgeP;Rowan,BrianG;Al-Dhaheri,Mariam;Williams,Christopher;Troup,Sandy;Begic,Sanela;Parisien,Michelle;Watson,PeterH;Murphy,LeighC
雌激素受体 α (ERα) 活性受多个位点磷酸化的调节。最近，几种针对 ERα 内各个磷酸化位点的特异性抗体已上市。此类抗体可能提供宝贵的工具，以深入了解磷酸化 ERα 在人乳腺肿瘤中的体内相关性。然而，首先需要对这些抗体进行免疫组织化学验证。在这项研究中，我们研究了针对 ERα 内特定磷酸化位点生成的几种抗体对于福尔马林固定、石蜡包埋的人乳腺癌活检样本的免疫组织化学的有用性。此外，这些数据首次证明，在乳腺癌中检测到多种磷酸化 ERα 形式（P-S104/106-ERα、P-S118-ERα、P-S167-ERα、P-S282-ERα、P-S294-ERα、P-T311-ERα 和 P-S559-ERα），这表明磷酸化 ERα 的谱分析的可能性同种型可能有助于选择从内分泌治疗中受益的乳腺癌患者亚组。
Estrogen receptor α (ERα) activity is regulated by phosphorylation at several sites. Recently several antibodies specific for individual phosphorylated sites within ERα have became available. Such antibodies potentially provide invaluable tools to gain insight into the relevance in vivo of phosphorylated ERα in human breast tumors. However, validation of these antibodies for immunohistochemistry in particular is necessary in the first instance. In this study we have investigated the usefulness of several antibodies generated to specific phosphorylated sites within ERα for immunohistochemistry of formalin-fixed, paraffin-embedded human breast cancer biopsy samples. As well, these data demonstrate for the first time, the detection of multiple phosphorylated ERα forms in breast cancer (P-S104/106-ERα, P-S118-ERα, P-S167-ERα, P-S282-ERα, P-S294-ERα, P-T311-ERα, and P-S559-ERα) suggesting the possibility that profiling of phosphorylated ERα isoforms might be useful in selecting subgroups of breast cancer patients that would benefit from endocrine therapy.