The number of domains in the ribosomal protein S1 as a hallmark of the phylogenetic grouping of bacteria
The number of domains in the ribosomal protein S1 as a hallmark of the phylogenetic grouping of bacteria
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DOI：
10.1371/journal.pone.0221370
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发表时间：
2019-08-22
期刊：
影响因子：
3.7
通讯作者：
Galzitskaya, Oxana V.
中科院分区：
文献类型：
--
作者：
Machulin, Andrey V.;Deryusheva, Evgenia I.;Galzitskaya, Oxana V.
核糖体蛋白 S1 家族约占所有细菌蛋白（包括 S1 结构域）的 20%。该家族的一个重要特征是细菌中结构域的多个拷贝，其数量在严格限制的范围内变化，从一到六个。在这项研究中，对 S1 的 1453 个序列进行自动详尽分析，使我们能够证明 S1 中的结构域数量是主要门中系统发育细菌分组的独特特征。根据命名法中的原核生物名称列表，在 30 个不同门中的 25 个门中鉴定出了 1453 个 S1 序列。所有记录中约 62% 被鉴定为六结构域 S1 蛋白，属于变形菌门。四结构域 S1 主要存在于厚壁菌门和放线菌门的蛋白质中。属于这些门的记录占所有记录的 33%。最少的两域 S1 大约占所有记录的 0.6%。最具代表性的四结构域和六结构域S1的第三和第四结构域与来自多核苷酸磷酸化酶的S1结构域和来自单结构域S1的S1结构域具有最高百分比的同一性。此外，对于这些基团，S1 的中心部分（第三个结构域）比末端结构域更加保守。
The family of ribosomal proteins S1 contains about 20% of all bacterial proteins including the S1 domain. An important feature of this family is multiple copies of structural domains in bacteria, the number of which changes in a strictly limited range from one to six. In this study, the automated exhaustive analysis of 1453 sequences of S1 allowed us to demonstrate that the number of domains in S1 is a distinctive characteristic for phylogenetic bacterial grouping in main phyla. 1453 sequences of S1 were identified in 25 out of 30 different phyla according to the List of Prokaryotic Names with Standing in Nomenclature. About 62% of all records are identified as six-domain S1 proteins, which belong to phylum Proteobacteria. Four-domain S1 are identified mainly in proteins from phylum Firmicutes and Actinobacteria. Records belonging to these phyla are 33% of all records. The least represented two-domain S1 are about 0.6% of all records. The third and fourth domains for the most representative four- and six-domain S1 have the highest percentage of identity with the S1 domain from polynucleotide phosphorylase and S1 domains from one-domain S1. In addition, for these groups, the central part of S1 (the third domain) is more conserved than the terminal domains.