Proteome of human minor salivary gland secretion
Proteome of human minor salivary gland secretion
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DOI：
10.1177/154405910808700508
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发表时间：
2008-05-01
影响因子：
7.6
通讯作者：
Oppenheim, F. G.
中科院分区：
文献类型：
--
作者：
Siqueira, W. L.;Salih, E.;Oppenheim, F. G.
最近口腔生物学的研究工作导致了人类主要唾液分泌物和整个唾液的蛋白质组的阐明。有人可能假设，与腮腺或颌下/舌下分泌物的蛋白质组相比，小腺分泌物的蛋白质组可能显示出明显不同的特征。为了验证这一假说，我们对小唾液腺分泌物的蛋白质组进行了首次探索。取健康志愿者的少量腺体分泌物，用高效液相色谱-电喷雾电离-串联质谱仪对其成分进行分析。这导致了56种蛋白质的鉴定，其中12种从未在任何唾液分泌物中鉴定出来。小涎腺分泌物蛋白质组的独特特征与其存在的种类和数量有关。唾液蛋白质组之间的差异对于特定的口腔功能可能是重要的。
Recent research efforts in oral biology have resulted in elucidation of the proteomes of major human salivary secretions and whole saliva. One might hypothesize that the proteome of minor gland secretions may show significantly different characteristics when compared with the proteomes of parotid or submandibular/sublingual secretions. To test this hypothesis, we conducted the first exploration into the proteome of minor salivary gland secretion. Minor gland secretion was obtained from healthy volunteers, and its components were subjected to liquid chromatography electrospray-ionization- tandem mass spectrometry. This led to the identification of 56 proteins, 12 of which had never been identified in any salivary secretion. The unique characteristics of the minor salivary gland secretion proteome are related to the types as well as the numbers of components present. The differences between salivary proteomes may be important with respect to specific oral functions.