The Western Blot
The Western Blot
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DOI：
10.1007/978-1-4939-2742-5_9
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Hnasko, Robert M.
中科院分区：
文献类型：
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作者：
Hnasko, Thomas S.;Hnasko, Robert M.
Western blotting是一种通过凝胶电泳分离蛋白质，将其印迹或转移到膜上，并对固定抗原进行选择性免疫检测的技术。这是一种重要的常规蛋白质分析方法，依赖于抗体-抗原相互作用的特异性，对于从复杂混合物中定性或半定量鉴定特定蛋白质及其分子量非常有用。本章将概述必要的步骤，包括蛋白质样品的凝胶电泳，将蛋白质从凝胶转移到膜载体，以及靶抗原的免疫检测。
Western blotting is a technique that involves the separation of proteins by gel electrophoresis, their blotting or transfer to a membrane, and selective immunodetection of an immobilized antigen. This is an important and routine method for protein analysis that depends on the specificity of antibody-antigen interaction and is useful for the qualitative or semiquantitative identification of specific proteins and their molecular weight from a complex mixture. This chapter will outline the requisite steps including gel electrophoresis of a protein sample, transfer of protein from a gel to a membrane support, and immunodetection of a target antigen.