Chromosome 1 open reading frame 10 (C1orf10) gene is frequently down-regulated and inhibits cell proliferation in oral squamous cell carcinoma
Chromosome 1 open reading frame 10 (C1orf10) gene is frequently down-regulated and inhibits cell proliferation in oral squamous cell carcinoma
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DOI：
10.1016/j.biocel.2005.02.005
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发表时间：
2005-08-01
影响因子：
4
通讯作者：
Tanzawa, H
中科院分区：
文献类型：
--
作者：
Imai, FL;Uzawa, K;Tanzawa, H
1号染色体开放阅读框10（C1 orf 10）是最近发现的一个编码5100 EF-手形钙结合基序的蛋白质的基因，已知其在食管鳞状细胞癌中表达下调。为了探讨C1 orf 10基因功能的缺失是否与口腔鳞状细胞癌（OSCC）的发生发展有关，本研究采用逆转录聚合酶链反应（RT-PCR）和实时荧光定量PCR技术检测了C1 orf 10基因在口腔鳞状细胞癌（OSCC）中的表达。不仅在OSCC衍生的细胞系中，而且在肿瘤组织中检测到C1 orf 10基因的高频率降低。接下来，为了确定该基因在口腔癌发生中的生物学功能，我们将C1 orf 10与蜕皮激素诱导系统转染到OSCC细胞系中，并分析其过表达的影响。C1 orf 10表达的诱导导致细胞增殖速率显着下降，细胞周期停滞在G期，细胞周期蛋白D1表达下调。然而，我们无法检测到凋亡细胞百分比的显著差异。因此，我们的研究结果表明，C1 orf 10基因的下调在口腔癌的发生中起作用，其表达可能通过抑制细胞周期负调控OSCC细胞增殖。(C)2005爱思唯尔有限公司保留所有权利。
Chromosome 1 open reading frame 10 (C1orf10) is a recently identified gene encoding a protein with an 5100 EF-hand calcium-binding motif, and its expression is known to be down-regulated in esophageal squamous cell carcinoma. In this study, to determine whether the loss of C1orf10 gene function could contribute to the development of oral squamous cell carcinoma (OSCC), we have evaluated the expression status of this gene by reverse transcriptase-polymerise chain reaction (RT-PCR) analysis and quantitative real-time PCR analysis. A high frequency of decrease in C1 orf10 gene was detected not only in OSCC-derived cell lines but also in tumor tissues. Next, to define biological function of this gene in oral carcinogenesis, we transfected C1orf10 with an Ecdysone-inducible system in OSCC cell lines and analyzed the effects of its overexpression. Induction of C1orf10 expression resulted in a significant decline in the rate of cell proliferation, and in an arrest in the G, phase of the cell cycle, with a down-regulation of Cyclin D1 expression. However, we could not detect significant difference in the percentage of apoptotic cells. Thus, our results suggest that the down-regulation of C1 orf10 gene plays a role in oral carcinogenesis, and that its expression may negatively regulate OSCC cell proliferation by arresting the cell cycle. (C) 2005 Elsevier Ltd. All rights reserved.