Metal binding by pyridine-2,6-bis(monothiocarboxylic acid), a biochelator produced by Pseudomonas stutzeri and Pseudomonas putida
Metal binding by pyridine-2,6-bis(monothiocarboxylic acid), a biochelator produced by Pseudomonas stutzeri and Pseudomonas putida
复制标题
吡啶-2,6-双（单硫代羧酸）与金属的结合，吡啶-2,6-双（单硫代羧酸）是施氏假单胞菌和恶臭假单胞菌产生的生物螯合剂
DOI：
10.1023/a:1015067729660
复制
发表时间：
2004
期刊：
影响因子：
3.6
通讯作者：
R. Crawford
中科院分区：
文献类型：
--
作者：
Jason C. Stolworthy;A. Paszczynski;R. Korus;R. Crawford
合成了一种促进四氯化碳降解的天然金属螯合剂吡啶-2,6-二（单硫代羧酸）（pdtc），并用电位法和分光光度法对其进行了研究。pdtc连续加成的前两个质子常数（pK）分别为5.48和2.58。第三步质子化常数估计为1.3。对铁（III）、镍（II）和钴（III）的稳定性（亲和力）常数采用电位滴定法或分光光度法测定。结果表明，pdtc对Fe（III）具有较强的亲和力，对其他金属也具有类似的亲和力。Co(pdtc)21-的稳定常数（log K）为33.93；Fe(pdtc)21-为33.36；Ni(pdtc)22-为33.28。这些质子常数和高亲和力常数表明，在生理pH范围内，铁pdtc配合物在已知的细菌螯合剂中具有铁结合的最高有效稳定性常数之一。
Pyridine-2,6-bis(monothiocarboxylic acid) (pdtc),a natural metal chelator produced by Pseudomonas stutzeri and Pseudomonas putidathat promotes the degradation of carbon tetrachloride, was synthesized and studiedby potentiometric and spectrophotometric techniques. The first two stepwise protonationconstants (pK) for successive proton addition to pdtc were found to be 5.48 and2.58. The third stepwise protonation constant was estimated to be 1.3. The stability (affinity)constants for iron(III), nickel(II), and cobalt(III) were determined by potentiometric orspectrophotometric titration. The results show that pdtc has strong affinity for Fe(III)and comparable affinities for various other metals. The stability constants (log K) are 33.93 for Co(pdtc)21-; 33.36 for Fe(pdtc)21-; and 33.28 for Ni(pdtc)22-. These protonationconstants and high affinity constants show that over a physiological pH range theferric pdtc complex has one of the highest effective stability constants for ironbinding among known bacterial chelators.