Drug localisation and growth inhibition studies of vindesine-monoclonal anti-CEA conjugates in a human tumour xenograft
Drug localisation and growth inhibition studies of vindesine-monoclonal anti-CEA conjugates in a human tumour xenograft
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长春地辛单克隆抗 CEA 缀合物在人肿瘤异种移植物中的药物定位和生长抑制研究
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
W. Smith
中科院分区：
文献类型：
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作者：
G. Rowland;R. G. Simmonds;V. Gore;C. Marsden;W. Smith
研究了氚化长春地辛（3 H-VDS）在荷人结肠直肠肿瘤异种移植物的无胸腺小鼠的组织和肿瘤中的分布。当3 H-VDS作为与单克隆抗CEA抗体（11.285.14）的偶联物注射时，获得了选择性肿瘤定位，但不作为与非结合性单克隆IgG 1（Ag 8）的偶联物或游离琥珀酰-VDS注射。在较宽的剂量范围内，注射3 H-VDS-11.285.14后肿瘤中定位的VDS量与注射量成比例增加。与正常组织相比，使用11.285.14的Fab片段制备的缀合物没有显示出选择性肿瘤摄取的证据。在1.5 mg/kg游离VDS和2.5 mg/kg结合VDS下，生长抑制率为50%。然而，这种结合物的毒性要小得多。
SummaryThe distribution of tritiated vindesine (3H-VDS) was studied in the tissues and tumours of athymic mice bearing a human colorectal tumour xenograft. Selective tumour localisation was obtained when 3H-VDS was injected as a conjugate with a monoclonal anti-CEA antibody (11.285.14) but not as a conjugate with a non-binding monoclonal IgG1 (Ag8) or as free succinoyl-VDS. The amounts of VDS that localised in the tumour following injections of 3H-VDS-11.285.14 increased in proportion to the amount injected, over a wide dose range. Conjugates prepared using the Fab fragments of 11.285.14 showed no evidence of selective tumour uptake in comparison with normal tissues.Various dose levels of VDS-11.285.14 conjugate and free VDS were studied for effects on the growth of the tumour xenograft. A growth inhibition of 50% was obtained at 1.5 mg/kg with free VDS and at 2.5 mg/kg with conjugated VDS. The conjugate was, however, considerably less toxic.
通过单克隆抗体对人类肿瘤异种移植物进行放射免疫检测。
影响因子：
11.2
作者：
Herlyn,D;Powe,J;Alavi,A;Mattis,JA;Herlyn,M;Ernst,C;Vaum,R;Koprowski,H
通讯作者：
Koprowski,H