Expressional divergence of the fatty acid-amino acid conjugate-hydrolyzing aminoacylase 1 (LACY-1) in Helicoverpa armigera and Helicoverpa assulta
Expressional divergence of the fatty acid-amino acid conjugate-hydrolyzing aminoacylase 1 (LACY-1) in Helicoverpa armigera and Helicoverpa assulta
复制标题
棉铃虫和苏尔塔中脂肪酸-氨基酸缀合物水解氨酰化酶 1 (L-ACY-1) 的表达差异
DOI：
10.1038/s41598-017-09185-2
复制
发表时间：
2017-08-18
影响因子：
4.6
通讯作者：
Li, Xianchun
中科院分区：
文献类型：
--
作者：
Cheng, Qian;Gu, Shaohua;Li, Xianchun
生产FACs的通才和专业食草动物如何调节其FACs水解酶L-ACY-1以平衡FACs的有益作用与有害作用仍然是未知的。为了解决这个问题，我们比较了L-ACY-1的表达在棉铃虫和烟青虫，一对密切相关的同胞物种不同，主要是在其宿主范围内，由相同的宿主植物，蛋白质，可消化的碳水化合物（P：C）的比例，或化感物质。L-ACY-1在H.棉铃虫对棉铃虫的抗性受辣椒果实的诱导而受棉铃的抑制。assulta.供试寄主植物的代表性化感物质（辣椒素）显著或不显著诱导H.在棉铃虫中，辣椒素和棉酚对烟碱的诱导或抑制作用均不显著。assulta. L-ACY-1表达在平衡（P50：C50和P53：C47）或偏向蛋白质的饮食中保持低水平/不变，并在偏向碳水化合物的饮食中诱导。棉铃虫在平衡日粮中的水平最高，而在偏重蛋白质或碳水化合物的日粮中则有所降低。assulta. L-ACY-1在H. assulta比H.棉铃虫的大多数喂养治疗。这种表达的分歧表明，FACs主要用于去除过多的氮在通才，但在专家的氮同化。
How FACs-producing generalist and specialist herbivores regulate their FACs-hydrolyzing enzyme L-ACY-1 to balance FACs' beneficial vs. detrimental effects remains unknown. To address this question, we compared L-ACY-1 expression in Helicoverpa armigera and Helicoverpa assulta, a pair of closely related sibling species differing mainly in their host range, by the same sets of hostplants, protein to digestible carbohydrate (P:C) ratios, or allelochemical. L-ACY-1 expression remained low/unchanged in H. armigera, but was induced by hot pepper fruits and repressed by cotton bolls in H. assulta. The representative allelochemicals of the tested hostplants significantly (capsaicin) or insignificantly (gossypol and nicotine) induced L-ACY-1 expression in H. armigera, but insignificantly inhibited (capsaicin and gossypol) or induced (nicotine) it in H. assulta. L-ACY-1 expression remained low/unaltered on balanced (P50:C50 and P53:C47) or protein-biased diets and induced on carbohydrate-biased diets in H. armigera, but was at the highest level on balanced diets and reduced on either protein-or carbohydrate-biased diets in H. assulta. Furthermore, L-ACY-1 expression was significantly higher in H. assulta than in H. armigera for most of feeding treatments. Such expressional divergences suggest that FACs are utilized mainly for removal of excessive nitrogen in generalists but for nitrogen assimilation in specialists.