GC-MS based metabolomics study of stems and roots of Ephedra sinica.
GC-MS based metabolomics study of stems and roots of Ephedra sinica.
复制标题
DOI：
10.1016/j.jpba.2015.04.035
复制
发表时间：
2015-10
影响因子：
3.4
通讯作者：
Mengying Lv;Jianbo Sun;Min Wang;Wan-qiu Huang;Hongyan Fan;Fengguo Xu;Zunjian Zhang
中科院分区：
文献类型：
--
作者：
Mengying Lv;Jianbo Sun;Min Wang;Wan-qiu Huang;Hongyan Fan;Fengguo Xu;Zunjian Zhang
中药因其解剖部位或炮制方法的不同，其疗效差异很大，麻黄（Ephedrasinica，ES）即是一例。为了更好地了解ES的茎（称为麻黄，MH）和根（称为麻黄根，MHG）的不同传统用途，应调查其治疗物质基础。在这项研究中，麻黄碱生物碱与初级代谢产物同时使用GC-MS代谢组学分析。麻黄碱（Ephedrine，E）是MH中治疗哮喘的主要活性成分。结果表明，与MH相比，MHG中5种麻黄碱类生物碱的含量较低，这可能是MHG尚未被用作平喘药的原因。此外，这些重要的麻黄碱生物碱表现出强的正相关性与五个初级代谢产物。总之，这项研究有助于更好地了解MH和MHG的不同传统用途。
Therapeutic effects of herbal medicines differ greatly due to the use of different anatomical parts or processing methods in traditional Chinese medicine, andEphedra sinica(ES) is just a case in point. To better understand different traditional uses of the stems (known as Mahuang, MH) and roots (known as Mahuanggen, MHG) of ES, their therapeutic material basis should be investigated. In this study, ephedrine alkaloids were profiled simultaneously with primary metabolites using GC–MS based metabolomics. Ephedrine (E) has been reported to be the major bioactive constituent in MH for the treatment of asthma. The results showed that compared with MH, MHG contained much lower levels of five ephedrine alkaloids, which may well explain that MHG has not been used as an antiasthmatic. Additionally, these pharmacologically important ephedrine alkaloids exhibited strong positive correlation with five primary metabolites. In conclusion, this study facilitates better understanding of different traditional uses of MH and MHG.