TAS1 trans-Acting siRNA Targets Are Differentially Regulated at Low Temperature, and TAS1 trans-Acting siRNA Mediates Temperature-Controlled At1g51670 Expression
TAS1 trans-Acting siRNA Targets Are Differentially Regulated at Low Temperature, and TAS1 trans-Acting siRNA Mediates Temperature-Controlled At1g51670 Expression
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DOI：
10.1271/bbb.100111
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发表时间：
2010-07-01
影响因子：
1.6
通讯作者：
Saitoh, Yasushi
中科院分区：
文献类型：
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作者：
Kume, Kohei;Tsutsumi, Ken-ichi;Saitoh, Yasushi
为了耐受相当大的温度波动，植物需要对温度控制的基因表达进行精确调控。本研究探讨了TAS1反式作用siRNA(TasiRNA)在拟南芥温控基因表达中的作用。在4℃时，TAS1 tasiRNA的积累被下调。随着TAS1 tasiRNA介导的切割减少，TAS1 tasiRNA的靶标At1g51670在4℃时表达上调，但在Dicer样酶(DCL)4突变体(dcl4-2)中表达上调，这在tasiRNA的生物发生中受到损害。另外两个TAS1 tasiRNA靶标At4g29760和At5g18040在野生型和dcl4-2中的表达都在4℃上调。然而，在将温度转移到22℃后，低温诱导的At4g29760在野生型中的表达迅速下降，但在dcl4-2中没有。因此，TAS1 tasiRNA起到了清除过量AT4g29760转录本的作用。我们的数据表明，TAS1 tasiRNA靶标的差异调控参与了温度控制的基因表达。
To endure considerable fluctuations in temperature, plants need precise regulation of temperature-controlled gene expression. In this study, the involvement of TAS1 trans-acting siRNA (tasiRNA) in temperature-controlled gene expression was examined in Arabidopsis. The accumulation of TAS1 tasiRNA was downregulated at 4 degrees C. Concomitant with the reduction of TAS1 tasiRNA-mediated cleavage, expression of At1g51670, a target of TAS1 tasiRNA, was upregulated at 4 degrees C in the wild type but not in a dicer-like enzyme (DCL) 4 mutant (dcl4-2), which is impaired in tasiRNA biogenesis. The expression of At4g29760 and of At5g18040, further TAS1 tasiRNA targets, was upregulated both in the wild type and in dcl4-2 at 4 degrees C. However, after shifting the temperature to 22 degrees C, low-temperature-induced expression of At4g29760 rapidly dropped in the wild type, but not in dcl4-2. Thus TAS1 tasiRNA acted as a sweeper for the clearance of excess amounts of At4g29760 transcripts. Our data suggest that differential regulation of TAS1 tasiRNA targets is involved in temperature-controlled gene expression.