Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella
Intra-Amoeba Multiplication Induces Chemotaxis and Biofilm Colonization and Formation for Legionella
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DOI：
10.1371/journal.pone.0077875
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发表时间：
2013-10-24
期刊：
影响因子：
3.7
通讯作者：
Berjeaud, Jean-Marc
中科院分区：
文献类型：
--
作者：
Bigot, Renaud;Bertaux, Joanne;Berjeaud, Jean-Marc
嗜肺军团菌是一种兼性胞内细菌，是军团菌病的病原体。在环境中，这种致病细菌定植于生物膜以及阿米巴，这为军团菌的复制提供了丰富的环境。当接种在预形成的生物膜上时，L.嗜肺菌能够建立并存活，并且仅在生物膜的表面发现。不同的表型观察时，L。用于实现预形成生物膜或形成单种生物膜的嗜肺菌在实验室培养肉汤中培养或在阿米巴内细胞内生长。事实上，在变形虫体内发育的细菌，在沉积在固体表面时形成簇。此外，我们的研究结果表明，变形虫内的增殖增加了L。pneumophila产生多糖，因此增强了其建立生物膜的能力。最后，研究了L.嗜肺菌可能与趋化性分子物质的分泌有关。
Legionella pneumophila, a facultative intracellular bacterium, is the causative agent of legionellosis. In the environment this pathogenic bacterium colonizes the biofilms as well as amoebae, which provide a rich environment for the replication of Legionella. When seeded on pre-formed biofilms, L. pneumophila was able to establish and survive and was only found at the surface of the biofilms. Different phenotypes were observed when the L. pneumophila, used to implement pre-formed biofilms or to form mono-species biofilms, were cultivated in a laboratory culture broth or had grown intracellulary within the amoeba. Indeed, the bacteria, which developed within the amoeba, formed clusters when deposited on a solid surface. Moreover, our results demonstrate that multiplication inside the amoeba increased the capacity of L. pneumophila to produce polysaccharides and therefore enhanced its capacity to establish biofilms. Finally, it was shown that the clusters formed by L. pneumophila were probably related to the secretion of a chemotaxis molecular agent.