The Transform Class in SPARX and EMAN2
The Transform Class in SPARX and EMAN2
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DOI：
10.1016/j.jsb.2006.06.002
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发表时间：
2007-01-01
影响因子：
3
通讯作者：
Penczek, Pawel A.
中科院分区：
文献类型：
--
作者：
Baldwin, P. R.;Penczek, Pawel A.
我们描述了旨在处理二维数据和三维数据的刚体运动的SPARX/EMAN2中的转换类别。我们描述了不同电子显微镜软件包中使用的欧拉角惯例之间的关系，并提供了简单脚本的示例，这些脚本在这些软件包之间执行Eulerian角度转换并处理其他相关任务。转换类还负责生成点组对称性操作以及在球体上生成准时分布点（用于创建单个粒子重建中的结构细化过程的参考投影）。我们讨论了如何在代码内部进行此操作，以及如何通过SPARX Interactive接口访问对称操作。我们介绍了班级实现的所有点组对称对称对称性的对称操作的全面描述。最后，我们提供有关与旋转操作相关的许多典型问题的解决方案 - 双轴断层扫描，不对称亚基的描述，投影方向的准均匀分布等，并提供了如何使用操作解决这些问题的示例在转换类中。 （c）2006 Elsevier Inc.保留所有权利。
We describe the Transform Class in SPARX/EMAN2 that is designed to handle rigid body motions of two- and three-dimensional data. We describe relationships between Eulerian angles conventions used in different electron microscopy software packages, as well as give examples of the simple scripts that execute conversions of Eulerian angles between these packages and handle other related tasks. The Transform Class is also responsible for generating point-group symmetry operations as well as generating quasi-evenly distributed points on the sphere (which is used in creating reference projections for structure refinement procedures in single particle reconstruction). We discuss how this is carried out internally in the code, and how symmetry operations are accessed through the SPARX interactive interface. We present a comprehensive description of symmetry operations for all point-group symmetries, as implemented in the class. Finally, we provide solutions to a number of typical problems associated with rotation operations-alignment of markers for dual-axis tomography, delineations of asymmetric subunits, quasi-uniform distribution of projection directions and such, and provide examples how these problems are solved using operations in the Transform Class. (c) 2006 Elsevier Inc. All rights reserved.