ROLE OF U6 SNRNA IN 5' SPLICE-SITE SELECTION
ROLE OF U6 SNRNA IN 5' SPLICE-SITE SELECTION
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DOI：
10.1126/science.8266100
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发表时间：
1993-12-24
期刊：
影响因子：
56.9
通讯作者：
SERAPHIN, B
中科院分区：
文献类型：
--
作者：
KANDELSLEWIS, S;SERAPHIN, B
在交联实验的基础上，提出了两个描述U6小核RNA(SnRNA)与内含子5‘剪接点相互作用的模型。在这里，我们证明了U6SnRNA中的一个保守序列与5‘剪接连接处的保守核苷酸形成碱基对，这种相互作用参与了5’剪接位点的选择。这些结果证明了U6SnRNA在剪接中的特定功能，并提示U6SnRNA在剪接位点选择过程中具有校对作用。提出了一个模型，在该模型中，这种新的相互作用，连同先前描述的U6 SnRNA、U2 SnRNA和前信使RNA之间的相互作用，将分支点定位在5‘剪接位点附近，用于催化第一个剪接步骤。
Two models describing the interaction between U6 small nuclear RNA (snRNA) and the 5' splice site of introns have been proposed on the basis of cross-linking experiments. Here it is shown that a conserved sequence present in U6 snRNA forms base pairs with conserved nucleotides at the 5' splice junction and that this interaction is involved in 5' splice site choice. These results demonstrate a specific function for U6 snRNA in splicing and suggest that U6 snRNA has a proofreading role during splice site selection. A model is presented in which this new interaction, in concert with previously described interactions between U6 snRNA, U2 snRNA, and the pre-messenger RNA, would position the branch point near the 5' splice site for the catalysis of the first splicing step.