α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.)
α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.)
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DOI：
10.1016/j.foodchem.2007.05.077
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发表时间：
2008-01-01
期刊：
影响因子：
8.8
通讯作者：
Kawabata, Jun
中科院分区：
文献类型：
--
作者：
Bhandari, Megh Raj;Jong-Anurakkun, Nilubon;Kawabata, Jun
草药已经被世界各地不同文化用于治疗糖尿病多年。尼泊尔草药Pakhanbhed是自史前时代以来用于糖尿病的传统疗法之一。在这项研究中，我们使用体外模型研究了抗糖尿病活性，并从Pakhanbhed中分离出活性化合物。提取物经分级分离得到两种活性成分，（-）-3-O-没食子儿茶素和（-）-3-O-没食子酰儿茶素，这两种成分均为首次从该植物中分离得到。这些分离的化合物对大鼠肠α-葡糖苷酶和猪胰α-淀粉酶表现出显著的剂量依赖性酶抑制活性。蔗糖、麦芽糖酶和α-淀粉酶对[（-）-3-O-没食子酰儿茶素]的IC 50值分别为560、334和739 μ M，对[（-）-3-O-没食子酰儿茶素]的IC 50值分别为297、150和401 μ M。我们的研究首次揭示了Pakhanbhed的抗糖尿病潜力，这项研究可能有助于开发治疗糖尿病和相关症状的药物制剂或营养和功能性食品。(c)2007爱思唯尔有限公司保留所有权利。
Herbal medicine has been used for many years by different cultures around the world for the treatment of diabetes. The Nepalese herb Pakhanbhed, is one of the traditional remedies used for diabetes since prehistoric times. In this study, we examined the anti-diabetic activity using an in vitro model and isolated the active compounds from Pakhanbhed. Extraction and fractionation of the extract lead to the isolation of two active compounds, (-)-3-O-galloylepicatechin and (-)-3-O-galloylcatechin and these are reported from this plant species for the first time. These isolated compounds demonstrated significant dose dependent enzyme inhibitory activities against rat intestinal alpha-glucosidase and porcine pancreatic alpha-amylase. IC50 value for sucrose, maltase and alpha-amylase were 560, 334 and 739 mu M, respectively for [(-)-3-O-galloylepicatechin] and 297, 150 and 401 mu M, respectively for [(-)-3-O-galloylcatechin]. Our study, for the first time, revealed the anti-diabetic potential of Pakhanbhed and this study could be helpful to develop medicinal preparations or nutraccutical and functional foods for diabetes and related symptoms. (c) 2007 Elsevier Ltd. All rights reserved.