Translational profiling: The genome-wide measure of the nascent proteome
Translational profiling: The genome-wide measure of the nascent proteome
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DOI：
10.1093/bfgp/3.2.103
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发表时间：
2004-08-01
期刊：
影响因子：
--
通讯作者：
Preiss, Thomas
中科院分区：
文献类型：
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作者：
Beilharz, Traude H.;Preiss, Thomas
真核细胞中的翻译在物理上和时间上都与转录分离。这为细胞提供了改变其蛋白质组的扩展选项：（1）直接，通过同步翻译与改变转录谱;（2）通过对转录组中已经存在的转录物施加改变的翻译控制;或（3）通过（1）和（2）的组合。在本文中，最近的研究结果在控制翻译的转录组使用微阵列分析。目前的技术和数据分析的指南也提供了，并在翻译控制的研究作为转录组和蛋白质组之间的接口的未来方向进行了概述。这项调查的重点是酵母菌，但所涵盖的主题具有普遍的相关性，在真核细胞中的翻译控制。
Translation in eukaryotic cells is both physically and temporally separated from transcription. This provides cells with extended options to alter their proteome: (1) directly, by synchronising translation with an altering transcriptional profile; (2) by imposing a changed translational control over transcripts already present in the transcriptome; or (3) by a combination of (1) and (2). In this paper, recent findings in the controlled translation of the transcriptome using microarray analyses are reviewed. A guide to the current technologies and data analysis is also provided, and future directions in the study of translational control as the interface between the transcriptome and the proteome are outlined. This survey is focused on the yeastSaccharomycescerevisiae, but the topics covered have universal relevance to the control of translation in eukaryotic cells.