Differential Expression of LIM-Homeodomain Factors in Cajal-Retzius Cells of Primates, Rodents, and Birds
Differential Expression of LIM-Homeodomain Factors in Cajal-Retzius Cells of Primates, Rodents, and Birds
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DOI：
10.1093/cercor/bhp242
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发表时间：
2010-08-01
期刊：
影响因子：
3.7
通讯作者：
Retaux, Sylvie
中科院分区：
文献类型：
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作者：
Abellan, Antonio;Menuet, Arnaud;Retaux, Sylvie
表达 Reelin 的 Cajal-Retzius (CR) 细胞是哺乳动物大脑皮层中最早产生的细胞之一，被认为对于端脑大脑皮层壁层状模式的发育和进化至关重要。 LIM-同源域 (LIM-hd) 转录因子在大脑发育过程中以高度受限和组合的方式表达，并且它们指定区域和细胞身份。我们研究了 LIM-hd 成员 Lhx1/Lhx2/Lhx5/Lhx6/Lhx9 在 reelin 表达细胞、大脑皮层和 CR 细胞起源区域（包括 3 种羊膜动物：小鼠、小鸡和猕猴的皮质下摆）中的表达。我们发现边缘区和下摆的组合 LIM-hd 表达存在主要差异。 1) Lhx5 是哺乳动物 CR 细胞的“优先 LIM-hd”，但这些细胞在雏鸡中不表达。 2) Lhx2 在小鸡的下摆表达，而在小鼠的该区域不表达。 3) 小鼠 CR 细胞表达 Lhx5/Lhx1，而猴子 CR 细胞则表达以下 4 个因子：Lhx1/Lhx2/Lhx5/Lhx9。我们从进化角度讨论了中线下摆和 CR 细胞类型的规范以及皮质层压模式的出现的进化结果。
Reelin-expressing Cajal-Retzius (CR) cells are among the earliest generated cells in the mammalian cerebral cortex and are believed to be crucial for both the development and the evolution of a laminated pattern in the pallial wall of the telencephalon. LIM-homeodomain (LIM-hd) transcription factors are expressed during brain development in a highly restricted and combinatorial manner, and they specify regional and cellular identity. We have investigated the expression of the LIM-hd members Lhx1/Lhx2/Lhx5/Lhx6/Lhx9 in the reelin-expressing cells, the pallium, and the regions of origin of CR cells including the cortical hem of 3 amniote species: the mouse, the chick, and the macaque monkey. We found major differences in the combinatorial LIM-hd expression in the marginal zone as well as in the hem. 1) Lhx5 is a "preferential LIM-hd" for CR cells in mammals but not expressed by these cells in chicks. 2) Lhx2 is expressed in the hem of the chick, whereas it is excluded from this region in mouse. 3) Whereas mouse CR cells express Lhx5/Lhx1, their monkey counterparts express 4 of these factors: Lhx1/Lhx2/Lhx5/Lhx9. We discuss our findings in evolutionary terms for the specification of the midline hem and CR cell type and the emergence of the cortical lamination pattern.