Predicted secondary structure similarity in the absence of primary amino acid sequence homology: hepatitis B virus open reading frames.
Predicted secondary structure similarity in the absence of primary amino acid sequence homology: hepatitis B virus open reading frames.
复制标题
在缺乏一级氨基酸序列同源性的情况下预测的二级结构相似性：乙型肝炎病毒开放阅读框。
DOI：
10.1073/pnas.81.9.2902
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发表时间：
1984
影响因子：
11.1
通讯作者：
Sninsky,JJ
中科院分区：
文献类型：
--
作者：
Schaeffer,E;Sninsky,JJ
进化上相关的蛋白质可能在一级氨基酸序列水平上有所分歧，同时保持相似的二级结构。计算机分析已用于在氨基酸序列水平上比较乙型肝炎病毒与土拨鼠肝炎病毒的开放阅读框，并预测推定多肽的相对亲水特性和二级结构。在不存在序列同源性的情况下，在相对亲水性和二级结构水平上可见相似性。这些数据强化了这些开放阅读框编码病毒蛋白的观点。这种类型的计算机分析可以更普遍地用于建立不具有明显序列同源性的蛋白质之间的结构相似性，以及评估开放阅读框是否是偶然的或编码蛋白质。
Proteins that are related evolutionarily may have diverged at the level of primary amino acid sequence while maintaining similar secondary structures. Computer analysis has been used to compare the open reading frames of the hepatitis B virus to those of the woodchuck hepatitis virus at the level of amino acid sequence, and to predict the relative hydrophilic character and the secondary structure of putative polypeptides. Similarity is seen at the levels of relative hydrophilicity and secondary structure, in the absence of sequence homology. These data reinforce the proposal that these open reading frames encode viral proteins. Computer analysis of this type can be more generally used to establish structural similarities between proteins that do not share obvious sequence homology as well as to assess whether an open reading frame is fortuitous or codes for a protein.