Biochemical differences in tumorigenic and nontumorigenic cells measured by Raman and infrared spectroscopy
Biochemical differences in tumorigenic and nontumorigenic cells measured by Raman and infrared spectroscopy
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DOI：
10.1117/1.1928050
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发表时间：
2005-05-01
影响因子：
3.5
通讯作者：
Freyer, JP
中科院分区：
文献类型：
--
作者：
Mourant, JR;Short, KW;Freyer, JP
红外和拉曼光谱都有潜力非侵入性地估计哺乳动物细胞的生化组成，尽管这不能从分析方法如峰分配或多变量分类方法中明确地确定。我们已经开发了一种拟合程序，该程序使用哺乳动物细胞中发现的主要类型的生化物质（蛋白质，DNA，RNA，脂质和糖原）的基础光谱来确定生化成分，该方法具有鲁棒性和可重复性。我们测量了两个红外和拉曼光谱的非致瘤性和致瘤性大鼠成纤维细胞系的活悬液。为了模拟体内条件，我们比较了非增殖的非致瘤细胞和增殖的致瘤细胞。使用两种光谱技术，发现两种非致瘤性/致瘤性细胞模型在生化组成上存在可重现的差异。这些包括在致瘤细胞中蛋白质和核酸的分数增加，脂质和糖原分数相应减少。在增殖和非增殖状态下对每种细胞类型的测量表明，增殖状态是振动光谱差异的主要决定因素，而不是致瘤性本身。与致瘤性相关的光谱变化的微小性可能是由于该系统中致癌变化的微妙性质（单个突变致癌基因）。(c)2005年，由光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）主办。
Both infrared and Raman spectroscopies have the potential to noninvasively estimate the biochemical composition of mammalian cells, although this cannot be unambiguously determined from analysis approaches such as peak assignment or multivariate classification methods. We have developed a fitting routine that determines biochemical composition using basis spectra for the major types of biochemicals found in mammalian cells (protein, DNA, RNA, lipid and glycogen), which is shown to be robust and reproducible. We measured both infrared and Raman spectra of viable suspensions of pairs of nontumorigenic and tumorigenic rat fibroblast cell lines. To model in vivo conditions, we compared nonproliferating, nontumorigenic cells to proliferating, tumorigenic cells. Reproducible differences in biochemical composition were found for both nontumorigenic/tumorigenic cell models, using both spectroscopic techniques. These included an increased fraction of protein and nucleic acids in the tumorigenic cells, with a corresponding decrease in lipid and glycogen fractions. Measurements of each cell type in both the proliferating and nonproliferating states showed that proliferative status was the major determinant of differences in vibrational spectra, rather than tumorigenicity per se. The smallness of the spectral changes associated with tumorgenicity may be due to the subtle nature of the oncogenic change in this system (a single mutant oncogene). (c) 2005 Society of Photo-Optical Instrumentation Engineers.