Antigen-specific stimulation of histamine releasing factors in diisocyanate-induced occupational asthma.
Antigen-specific stimulation of histamine releasing factors in diisocyanate-induced occupational asthma.
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二异氰酸酯诱发的职业性哮喘中组胺释放因子的抗原特异性刺激。
DOI：
10.1164/ajrccm.150.4.7522854
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发表时间：
1994
影响因子：
24.7
通讯作者：
Bernstein,DI
中科院分区：
文献类型：
--
作者：
Herd,ZL;Bernstein,DI
二异氰酸酯诱发的哮喘不同于由蛋白质过敏原引起的职业性哮喘（OA），因为特异性IgE抗体反应很少被识别。为了探讨二异氰酸酯哮喘的免疫发病机制，二异氰酸酯接触工人进行了评估，在体外生产抗原特异性单核细胞衍生的组胺释放因子（HRF）。OA患者对二异氰酸酯-HSA抗原的平均HRF反应显著高于二异氰酸酯暴露的无症状受试者（p < 0.05）。HRF和二异氰酸酯特异性抗体之间没有发现关联。外周血单个核细胞（PBMC）的亚群的HRF生产的分析表明，淋巴细胞和粘附细胞的自发和抗原刺激的HRF的主要来源。结果表明，由PBMC产生的抗原特异性HRF是二异氰酸酯诱导的哮喘的重要生物标志物。这是第一份半抗原特异性刺激PBMC导致HRF产生的报告。
Diisocyanate-induced asthma differs from occupational asthma (OA) caused by protein allergens in that specific IgE antibody responses are rarely identified. To investigate the immunopathogenesis of diisocyanate asthma, diisocyanate-exposed workers were evaluated for in vitro production of antigen-specific mononuclear cell-derived histamine releasing factor (HRF). The mean HRF response to diisocyanate-HSA antigens was significantly greater in patients with OA than in diisocyanate-exposed asymptomatic subjects (p < 0.05). No association was found between HRF and diisocyanate-specific antibodies. Analysis of HRF production by subpopulations of peripheral blood mononuclear cells (PBMC) showed that lymphocytes and adherent cells were major sources of both spontaneous and antigen-stimulated HRF. The results suggest that antigen-specific HRF produced by PBMCs are an important biomarker for diisocyanate-induced asthma. This is the first report of hapten-specific stimulation of PBMCs resulting in HRF production.