Wash-free and selective imaging of epithelial cell adhesion molecule (EpCAM) expressing cells with fluorogenic peptide ligands
Wash-free and selective imaging of epithelial cell adhesion molecule (EpCAM) expressing cells with fluorogenic peptide ligands
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DOI：
10.1016/j.bbrc.2018.04.059
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发表时间：
2018-06-02
影响因子：
3.1
通讯作者：
Uzawa, Takanori
中科院分区：
文献类型：
--
作者：
Bahadur, Tara K. C.;Suga, Kanako;Uzawa, Takanori
检测表达上皮细胞粘附分子 (EpCAM) 的细胞是识别血液中循环肿瘤细胞 (CTC) 的关键步骤。为了检测 EpCAM，我们设计并合成了一系列荧光肽。具体来说，我们通过将 EpCAM 结合肽 Ep114 的氨基酸替换为用环境敏感的 7-硝基-2,1,3-苯并恶二唑 (NBD-amPhe) 修饰的氨基苯丙氨酸，对其进行功能化。在六种合成的肽中，我们发现两种肽Q4X和V6X（X代表NBD-amPhe）保留了Ep114的结合能力，并且只需将这些肽添加到培养基中即可特异性标记EpCAM表达细胞。我们的免洗荧光肽配体将促进下一代 CTC 诊断设备的开发。 (C) 2018 Elsevier Inc. 保留所有权利。
Detection of the cells expressing an epithelial cell adhesion molecule (EpCAM) is a crucial step to identify circulating tumor cells (CTCs) from blood. To detect the EpCAM, we here designed and synthesized a series of fluorogenic peptides. Specifically, we functionalized an EpCAM-binding peptide, Ep114, by replacing its amino acids to an aminophenylalanine that was modified with environmentally sensitive 7-nitro-2,1,3-benzoxadiazole (NBD-amPhe). Among six synthesized peptides, we have found that two peptides, Q4X and V6X (X represents NBD-amPhe), retain the Ep114's binding ability and specifically mark EpCAM-expressing cells by just adding these peptides to the cultivation medium. Our wash-free, fluorogenic peptide ligands would boost the development of next generation devices for CTC diagnoses. (C) 2018 Elsevier Inc. All rights reserved.