Improvement of the CULTEX® exposure technology by radial distribution of the test aerosol
Improvement of the CULTEX® exposure technology by radial distribution of the test aerosol
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DOI：
10.1016/j.etp.2017.02.004
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发表时间：
2017-07-05
影响因子：
--
通讯作者：
Hochrainer, Dieter
中科院分区：
文献类型：
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作者：
Aufderheide, Michaela;Heller, Wolf-Dieter;Hochrainer, Dieter
在气液界面（ALI）的微孔膜上培养的基于细胞的系统的暴露已被认为是模拟呼吸道细胞暴露于天然空气传播物质的适当方法。这种暴露程序在稳定性和再现性方面的效率取决于细胞测试系统和暴露技术之间接口的最佳设计。实际的暴露系统有利于在专门设计的暴露装置中将空气中的物质动态引导至细胞表面。这些研究使用了两种模块类型，它们基于将测试气氛线性或径向馈送到测试系统。在我们的技术历史中，开发始于线性设计版本 CULTEX (R) 玻璃模块，满足运行 ALI 暴露研究的基本要求（Mohr 和 Durst，2005）。不同气氛向细胞分布的不稳定性促使我们创建了一种新的暴露模块，其特点是气溶胶向细胞的稳定且可重复的径向引导。结果就是 CULTEX RFS（Mohr 等人，2010）。在这项研究中，我们描述了两个系统在颗粒分布和沉积方面的差异，阐明了径向与线性气溶胶分布概念的优缺点。 (C) 2017 爱思唯尔有限公司。版权所有。
The exposure of cellular based systems cultivated on microporous membranes at the air-liquid interface (ALI) has been accepted as an appropriate approach to simulate the exposure of cells of the respiratory tract to native airborne substances. The efficiency of such an exposure procedure with regard to stability and reproducibility depends on the optimal design at the interface between the cellular test system and the exposure technique. The actual exposure systems favor the dynamic guidance of the airborne substances to the surface of the cells in specially designed exposure devices. Two module types, based on a linear or radial feed of the test atmosphere to the test system, were used for these studies. In our technical history, the development started with the linear designed version, the CULTEX (R) glass modules, fulfilling basic requirements for running ALI exposure studies (Mohr and Durst, 2005). The instability in the distribution of different atmospheres to the cells caused us to create a new exposure module, characterized by a stable and reproducible radial guidance of the aerosol to the cells. The outcome was the CULTEX RFS (Mohr et al., 2010). In this study, we describe the differences between the two systems with regard to particle distribution and deposition clarifying the advantages and disadvantages of a radial to a linear aerosol distribution concept. (C) 2017 Elsevier GmbH. All rights reserved.