Nkx2-1 and FoxE regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate Oikopleura dioica.
Nkx2-1 and FoxE regionalize glandular (mucus-producing) and thyroid-equivalent traits in the endostyle of the chordate Oikopleura dioica.
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Nkx2-1 和 FoxE 在脊索动物 Oikopleura dioica 的内样式中区域化腺体（产生粘液）和甲状腺等效特征。
DOI：
10.1016/j.ydbio.2021.05.021
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Sasakura Y and Ogasawara M*
中科院分区：
文献类型：
--
作者：
Onuma TA*;Nakanishi R;Sasakura Y and Ogasawara M*
内花柱是一种腹侧咽部器官，用于基础脊索动物的内部滤食，被认为与脊椎动物的滤泡甲状腺同源。它包含沿背腹(DV)轴排列的粘液产生区(腺体)和甲状腺等效区。虽然已知甲状腺相关基因(Nkx2-1、Foxe和甲状腺过氧化物酶(TPO))在内花柱中表达，但它们在器官内建立区域化中的作用尚未被证明。我们报道了Nkx2-1和Foxe是建立DV轴向同源性所必需的。基因组和表达分析表明，von Willebrand因子样(VWFL)和TPO/双氧化酶(DUOX)/Nkx2-1/FoxE分别是腺体和甲状腺相关基因的同源基因。基因敲除实验表明，Nkx2-1是腺体和甲状腺相关基因表达所必需的，而FOXE只对甲状腺相关基因是必需的。此外，在幼虫器官发生过程中，Nkx2-1的表达是FOXE表达所必需的。结果表明，Nkx2-1和Foxe在内花柱的区域化过程中起着重要的作用，包括(1)Nkx2-1依赖的腺区和(2)Nkx2-1/Foxe依赖的甲状腺等效区。DV轴的区域化可能负责沿DV轴组织咽部的腺性和甲状腺等效性征。
The endostyle is a ventral pharyngeal organ used for internal filter feeding of basal chordates and is considered homologous to the follicular thyroid of vertebrates. It contains mucus-producing (glandular) and thyroid-equivalent regions organized along the dorsoventral (DV) axis. Although thyroid-related genes (Nkx2-1,FoxE, andthyroid peroxidase (TPO))are known to be expressed in the endostyle, their roles in establishing regionalization within the organ have not been demonstrated. We report that Nkx2-1 and FoxE are essential for establishing DV axial identity in the endostyle ofOikopleura dioica. Genome and expression analyses showedvon Willebrand factor-like (vWFL)andTPO/dual oxidase (Duox)/Nkx2-1/FoxEas orthologs of glandular and thyroid-related genes, respectively. Knockdown experiments showed that Nkx2-1 is necessary for the expression of glandular and thyroid-related genes, whereas FoxE is necessary only for thyroid-related genes. Moreover, Nkx2-1 expression is necessary for FoxE expression in larvae during organogenesis. The results demonstrate the essential roles of Nkx2-1 and FoxE in establishing regionalization in the endostyle, including (1) the Nkx2-1-dependent glandular region, and (2) the Nkx2-1/FoxE-dependent thyroid-equivalent region. DV axial regionalization may be responsible for organizing glandular and thyroid-equivalent traits of the pharynx along the DV axis.