Oxyphor R2 and G2: phosphors for measuring oxygen by oxygen-dependent quenching of phosphorescence
Oxyphor R2 and G2: phosphors for measuring oxygen by oxygen-dependent quenching of phosphorescence
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DOI：
10.1016/s0003-2697(02)00384-6
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发表时间：
2002-11-15
影响因子：
2.9
通讯作者：
Wilson, DF
中科院分区：
文献类型：
--
作者：
Dunphy, I;Vinogradov, SA;Wilson, DF
氧依赖的磷光猝灭是一种有用的和基本上无创的光学方法，用于测量氧在体内和体外。磷光体的校准是绝对的，一旦磷光体在一个实验室中被校准，只要在相同的条件下进行测量，任何其他人都可以使用相同的常数。两个新的荧光粉，一个基于Pd-meso-四-（4-羧基苯基）卟啉和其他上的Pd-meso-四-（4-羧基苯基）四苯并卟啉，非常适合在体内氧测量。两种磷光体都是第2代聚谷氨酸Pd-卟啉-树枝状聚合物，在外层上具有16个羧酸酯基团。这些磷光体分别被指定为Oxyphor R2和Oxyphor G2。两者都高度可溶于生物流体如血浆中，并且它们穿透生物膜的能力非常低。对于Oxyphor R2，吸收光谱中的最大值在415和524 nm处，对于Oxyphor G2，吸收光谱中的最大值在440和632 nm处，而发射分别在700和800 nm附近。磷光体的校准常数在生理范围内（6.4至7.8）基本上与pH无关。通过使用Oxyphor G2非侵入性地确定大鼠皮下肿瘤生长中的氧分布来证明体内应用。(C)2002 Elsevier Science（美国）。All rights reserved.
Oxygen-dependent quenching of phosphorescence is a useful and essentially noninvasive optical method for measuring oxygen in vivo and in vitro. Calibration of the phosphors is absolute, and once phosphors have been calibrated in one laboratory the same constants can be used by anyone else as long as the measurement is done under the same conditions. Two new phosphors, one based on Pd-meso-tetra-(4-carboxyphenyl)porphyrin and the other on Pd-meso-tetra-(4-carboxyphenyl)tetrabenzoporphyrin, are very well suited to in vivo oxygen measurements. Both phosphors are Generation 2 polyglutamic Pd-porphyrin-dendrimers, bearing 16 carboxylate groups on the outer layer. These phosphors are designated Oxyphor R2 and Oxyphor G2, respectively. Both are highly soluble in biological fluids such as blood plasma and their ability to penetrate biological membranes is very low. The maxima in the absorption spectra are at 415 and 524 nm for Oxyphor R2 and 440 and 632 nm for Oxyphor G2, while emissions are near 700 and 800 nm, respectively. The calibration constants of the phosphors are essentially independent of pH in the physiological range (6.4 to 7.8). In vivo application is demonstrated by using Oxyphor G2 to noninvasively determine the oxygen distribution in a subcutaneous tumor growing in rats. (C) 2002 Elsevier Science (USA). All rights reserved.