High-throughput quantification of hydroxyproline for determination of collagen
High-throughput quantification of hydroxyproline for determination of collagen
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DOI：
10.1016/j.ab.2011.06.019
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发表时间：
2011-10-15
影响因子：
2.9
通讯作者：
Marshall, Susan
中科院分区：
文献类型：
--
作者：
Hofman, Kathleen;Hall, Bronwyn;Marshall, Susan
一种准确、高通量的胶原蛋白检测方法对于胶原蛋白产品的研究和开发至关重要。羟基脯氨酸是常规测定胶原定量提供测量。在测定前用酸水解和中和法制备样品所需的时间限制了目前测定羟脯氨酸的方法。这项工作描述了碱水解的条件，当与Woessner定义的比色法相结合时，提供了一种高通量，准确的羟脯氨酸测量方法。(C) 2011爱思唯尔公司版权所有。
An accurate and high-throughput assay for collagen is essential for collagen research and development of collagen products. Hydroxyproline is routinely assayed to provide a measurement for collagen quantification. The time required for sample preparation using acid hydrolysis and neutralization prior to assay is what limits the current method for determining hydroxyproline. This work describes the conditions of alkali hydrolysis that, when combined with the colorimetric assay defined by Woessner, provide a high-throughput, accurate method for the measurement of hydroxyproline. (C) 2011 Elsevier Inc. All rights reserved.