Seasonal Dynamics of Haptophytes and dsDNA Algal Viruses Suggest Complex Virus-Host Relationship.
Seasonal Dynamics of Haptophytes and dsDNA Algal Viruses Suggest Complex Virus-Host Relationship.
复制标题
DOI：
10.3390/v9040084
复制
发表时间：
2017-04-20
期刊：
影响因子：
--
通讯作者：
Sandaa RA
中科院分区：
文献类型：
--
作者：
Johannessen TV;Larsen A;Bratbak G;Pagarete A;Edvardsen B;Egge ED;Sandaa RA
病毒影响海洋浮游植物的生态和多样性。大多数浮游植物宿主与病毒相互作用的研究都集中在水华形成物种上，如赫氏艾米利亚藻或棕囊藻属。感染不形成明显水华的浮游植物的病毒的作用受到的关注较少。在这里，我们探索了浮游植物和藻类病毒在几个连续季节中的动态，重点关注普遍存在且多样化的浮游植物分支Haptophyta及其双链DNA病毒，它们可能具有感染haptophytes的能力。病毒和浮游植物的丰度和多样性表现出周期性的季节性变化，这主要是由水文条件解释的。通过 454 个标签测序，我们揭示了 93 个独特的附着植物操作分类单元 (OTU)，其季节性变化丰富。代表巨病毒科和藻DNA病毒科的61个独特病毒OTU与目前分离的藻类病毒仅表现出较远的关系。附着菌和病毒群落的组成和多样性全年变化很大，但不协调。少数病毒 OTU 在特定时间点高度丰富，表明与其宿主存在繁荣-萧条关系。大多数病毒OTU非常持久，这可能代表病毒与其宿主共存，或者能够利用多个宿主物种。
Viruses influence the ecology and diversity of phytoplankton in the ocean. Most studies of phytoplankton host–virus interactions have focused on bloom-forming species like Emiliania huxleyi or Phaeocystis spp. The role of viruses infecting phytoplankton that do not form conspicuous blooms have received less attention. Here we explore the dynamics of phytoplankton and algal viruses over several sequential seasons, with a focus on the ubiquitous and diverse phytoplankton division Haptophyta, and their double-stranded DNA viruses, potentially with the capacity to infect the haptophytes. Viral and phytoplankton abundance and diversity showed recurrent seasonal changes, mainly explained by hydrographic conditions. By 454 tag-sequencing we revealed 93 unique haptophyte operational taxonomic units (OTUs), with seasonal changes in abundance. Sixty-one unique viral OTUs, representing Megaviridae and Phycodnaviridae, showed only distant relationship with currently isolated algal viruses. Haptophyte and virus community composition and diversity varied substantially throughout the year, but in an uncoordinated manner. A minority of the viral OTUs were highly abundant at specific time-points, indicating a boom-bust relationship with their host. Most of the viral OTUs were very persistent, which may represent viruses that coexist with their hosts, or able to exploit several host species.